健骨固本胶囊对维甲酸所致实验性骨质疏松大鼠的影响  

Effect and mechanism of Jianguguben capsule on osteoporosis induced by retinoic acid in rats

作　　者：杨如会[1] 柏帅[1] 彭佼[1] 沈祥春[1] 

机构地区：[1]贵阳医学院药理研究室,贵阳550004

出　　处：《中华中医药杂志》2008年第11期1009-1011,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：贵州省中药现代化专项[No.黔科合农字(2005)5038]

摘　　要：目的:研究健骨固本胶囊对维甲酸所致大鼠实验性骨质疏松症的影响及可能作用机制。方法:通过灌胃维甲酸75mg·kg-1·d-1,连续2周复制大鼠骨质疏松模型,同时给予药物干预4周后,使用双能X线骨密度测定仪测定全身骨矿量(BMC)和全身骨密度(BMD)及股骨BMD;采用市售试剂盒分析血清抗酒石酸酸性磷酸酶(StrACP)活力和骨组织羟脯氨酸(HOP)含量;股骨三点弯曲实验分析最大应力、弹性模量和弹性能量吸收。结果:健骨固本胶囊可显著增加全身BMC,全身及股骨BMD;增加股骨干重指数;显著降低血清StrACP水平,提高骨HOP含量;提高骨最大应力、弹性模量和弹性能量吸收。结论:健骨固本胶囊对维甲酸所致大鼠实验性骨质疏松症模型具有显著的改善作用。objective： To investigate the effect and mechanism of Jianguguben capsule on osteoporosis induced by retinoic acid in rats. Methods： The rats were given retinoic acid 75mg（kg/d）for 2 weeks by intragastric administration, at the same time treated with different drugs according to various groups consecutive for 4 weeks. The bone mine density （BMD）and bone mine capacity （ BMC ） were assayed by double energy X-ray bone-densitometry equipment, the blood was collected to assay the activity of StrACP in serum, and the hydroxyproline（HOP） contents of femur by commercial kits, the bone＇s biomechanics of femur were detected. Results： Jianguguben capsule could significantly improve the bone＇s biomechanics, increase the BMD, BMC, index of dry femur＇ weight, the contents of HOP in femur significantly, and remarkable decrease the level of StrACP in serum of the osteoporotic rats induced by retinoic acid. Conclusion： Jiangguguben capsule has anti-osteoporotic effect on osteoporotic rats induced by retinoic acid.

关 键 词：骨质疏松症 健骨固本胶囊 维甲酸 中医药疗法 

分 类 号：R285.5[医药卫生—中药学] R274.91[医药卫生—中医学]