鸭源H5N1亚型禽流感病毒分离株的致病性及表面蛋白基因序列分析  

Pathogenicity of avian influenza viruse H5N1 from duck and sequence analysis of spike protein genes

作　　者：杨德全[1,2] 李雁冰[2] 施建忠[2] 陈化兰[2] 张书霞[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室农业部动物流感重点开放实验室国家禽流感参考实验室,黑龙江哈尔滨150001

出　　处：《畜牧与兽医》2008年第11期17-20,共4页Animal Husbandry & Veterinary Medicine

基　　金：农业部动物流感监测与防治专项项目;国家“十五”科技攻关项目(2004BA519A42);国家“十一五”科技支撑计划项目(2006BAD06A03)

摘　　要：分析1株2004年从广东省分离获得的HSN1型禽流感病毒株A／Duck／GuangdongJiedong／23／2004对SPF鸡和鸭的致病性，并对其血凝素（HA）基因和神经氨酸酶（NA）基因的序列进行测定，与GenBank中收录的其他序列进行比较。结果，此分离株对SPF鸡和鸭均具有高致病性，且致死率达100％。HA基因与SCK／ST／475／04的同源率最高，而NA基因与Ck／GD／178／04同源率最高。进化分析结果表明，此毒珠与DK／Chi—na／E319-2／03的HA、NA亲缘关系较近，推测它们来源于同一祖代毒株。推导的HA基因氨基酸裂解位点为-RRRKK-，具有典型高致病性禽流感的特征序列；NA基因颈部49～68位20个氨基酸缺失是近年来H5N1亚型优势流行株共同的遗传标志。Pathogenicity of one strain of Avian influenza virus （AIV） HSN1 named A/duck/GuangdongJiedong/23/2004 （ HSN1 ） （ DK/ GDJD/23/04）, was analyzed. The hemagglutin （HA） and neuraminidase （NA） genes had been sequenced and compared with those in GenBank. The results showed DK/GDJD/23/04 had high pathogenicity to the SPF chicken and duck, causing 100% mortality. DK/GDJD/ 23/04 shared high identity with SCK/ST/475/04 in HA gene and shared high identity with Ck/GD/178/04 in NA gene. Phylogenetic analysis revealed that both HA and NA genes of DK/GDJD/23/04 had genetically close relationship with DK/China/E319-2/03, which suggested that they came from the same origin. The sequence of cleavage site between HA1 and HA2 consisted of-RRRKK-, which was the sequence of the high pathogenic AIV, and a 20-amino acid delection in NA stalk （residues49-68）, was the genetic marker of the majority strains in recent years.

关 键 词：禽流感病毒 1-15N1亚型 致病性 血凝素 神经氨酸酶 序列分析 

分 类 号：S852.6[农业科学—基础兽医学]