冻干血小板功能的体外评价  

Evaluation of the function of lyophilized platelets

作　　者：周俊[1] 张长虹[1] 刘景汉[2] 杨连贵 杜海燕[1] 卢发强 

机构地区：[1]北京军区总医院输血科,北京100700 [2]解放军总医院输血科 [3]北京市通州区血站

出　　处：《中国输血杂志》2008年第10期774-777,共4页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金资助项目(编号:30271443)

摘　　要：目的评价冻干血小板的体外功能。方法将经过可逆性激活抑制、添加DMSO和海藻糖等低温保护剂、冷冻干燥后获得的血小板再水化,与新鲜血小板比较,应用流式仪检测分析其CD62p和PAC-1的表达,评价血小板活化状态和血小板反应能力;应用APACT检测诱导剂诱导的血小板最大聚集率并用SPAT评价血小板聚集和促凝血活性;应用扫描电镜和透射电镜技术研究血小板的形态和超微结构的变化。结果经过血小板激活抑制剂和冻干保护剂预处理的冻干血小板,再水化后对凝血酶的最大聚集率(79.0%)与对照组(86.2%)差异无统计学意义(P>0.05),对ADP和没食子酸诱导的聚集反应比对照分别降低49.34%和26.25%;经凝血酶激活后CD62p再表达率为50.88%略低于对照组70.32%(P<0.05),PAC-1再表达率为54.55%与对照组63.38%无明显差异;促凝血功能SPAT检测结果69.4 s与对照组70.6 s差异无统计学意义(P>0.05)。结论经过冷冻干燥保护剂和血小板激活抑制剂预处理后获得的冻干血小板,体外聚集活性和促凝血功能接近新鲜血小板。Objective To evaluate the in vitro function of lyophilized platelets which were treated with reversible activation-inhihitors,DMSO and trehalose, and then were frozen and desiccated. Methods After the lyophilization, platelets were rehydrated, expression of CD62p and PAC-1 and expressions of CD62p and Pac-1 induced by thromhin were measured as the indexes of platelet activation and reactivity. The maximal aggregation and SPAT were taken as the indexes of aggregation and pocoagulant function. Morphology and ultra structure of platelets were observed by Scanning and transmission Electron microscope. Results The thrombin induced maximal aggregation of the lyophilized platelets （ 79. 0% ） showed no significant difference from fresh platelets （86.2% ）, but the ADP or pmpylgallate induced aggregation of the lyophilized platelets were decreased by 49.34% and 26.25% , respectively. Thrombin induced CD62p re-expression of the lyophilized platelets was 50. 88 % ,lower than that of the control group, and PAC-1 was 54.55 %, similar to that of the control group. The procoagulant activity of the product had no significant difference from the control. Conclusion The aggregation and procoagulant functions of the recovered lyophilized platelets treated with reversible activation-inhihitors, DMSO and tmhalose are not significant different from fresh platelets.

关 键 词：血小板 冷冻干燥 海藻糖 DMSO CD62P PAC-1 

分 类 号：R331.143[医药卫生—人体生理学] R457.1[医药卫生—基础医学]