HBVcccDNA在乙型肝炎患者血清、外周单核细胞及肝组织中的分布  

HBVcccDNA of patients with hepatitis B in the serum, PBMC and liver tissue distribution

作　　者：张永乐[1] 徐岱[1] 吴晓星[1] 武静[1] 邵芯仪[1] 陈虹[1] 张勤[1] 

机构地区：[1]杭州市第六人民医院,310014

出　　处：《中华实验和临床病毒学杂志》2008年第5期342-344,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：杭州市科技局科研项目（2004433Q03）;杭州市卫生局重点传染专科课题

摘　　要：目的观察乙肝病毒共价闭合环状DNA（HBVcccDNA）在乙肝患者血清、外周单核细胞（PBMC）及肝组织中的分布情况。方法选取血清HBVDNA〉10^5拷贝／ml的乙肝患者50例，血清HBVDNA〈10^5拷贝／ml的乙肝患者30例，脂肪肝患者（非乙型肝炎）20例，同时采用实时荧光定量聚合酶链反应检测患者血清、PBMC及肝组织中HBVcccDNA的存在情况。结果50份血清HBVDNA〉10^5拷贝／ml的标本中血清HBVcccDNA检出28例，检出率56％，PBMC HBVcccDNA检出29例，检出率58％，肝组织中HBVcccDNA检出44例，检出率88％，血清、PBMC的检出较肝组织检出差异有统计学意义P〈0．005，血清较PBMC检出差异无统计学意义P〉0．005。30份血清HBVDNA〈10^5拷贝／ml的标本中血清、PBMC HBVcccDNA检出均为2例，检出率6．67％，肝组织中HBVcccDNA检出6例，检出率20％，血清、PBMC、肝组织三者之间检出差异无统计学意义，P〉0．005。20份脂肪肝患者的血清、PBMC及肝组织中均未检出HBVcccDNA。结论HBVcccDNA主要存在于乙型肝炎患者的肝脏中，乙肝患者血清及PBMC中也有HBVcccDNA的存在但较肝组织中要少的多。Objective Hepatitis B virus covalently closed circular DNA in the serum of patients with hepatitis B, peripheral mononuclear cells （PBMC） and liver tissue distribution. Methods Serum HBV DNA 〉 105 copies/ml 50 cases of hepatitis B patients, serum HBV DNA 〈 105 copies/ml 30 cases of hepatitis B patients, patients with fatty liver （hepatitis B） 20 eases, using real-time quantitative polymerase chain reaction for detection of serum, and liver tissue in PBMC HBVcccDNA the existence. Results Of 50 serum HBV DNA 〉 105 copies/ml cecDNA the serum specimens were 28 cases, 56% detection rate, 29 cases were PBMC HBVcccDNA, 58% detection rate, liver tissue HBVcccDNA were 44 cases, 88% detection rate, serum, the PBMC were detected in liver tissue were significant differences in P 〈 0.005, compared serum PBMC were no significant differences in P 〉0.005. 30 serum HBV DNA 〈 105copies/ml the serum specimens, PBMC eccDNA detected two cases were 6.67% detection rate, liver tissue cccDNA were six cases 20% detection rate, serum, PBMC, were among the liver tissue was not significantly different P 〉 0.005. 20 in the serum of patients with fatty liver, liver tissue in PBMCwere not detected HBVcccDNA. Conclusion HBVcccDNA mainly in the liver of patients with hepatitis B, hepatitis B patients and also cccDNA PBMC in the presence of liver tissue but a few of many.

关 键 词：单核细胞 肝 DNA 

分 类 号：R512.6[医药卫生—内科学] R450[医药卫生—临床医学]