利用SSR-BSA技术筛选玉米粗缩病抗性基因分子标记  

Identification of the Molecular Markers Linked to the MRDV-resistance Locus in Maize Using SSR-BSA Technique

作　　者：陈艳萍[1] 孟庆长[1] 袁建华[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏南京210014

出　　处：《江苏农业学报》2008年第5期590-594,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省自然科学基金项目(BK2005106);国家科技支撑计划项目(2006BAD01A03);江苏省农业科技自主创新资金项目[CX(07)101]

摘　　要：采用网棚集团接种法,对感病自交系苏951和抗病自交系87-1的P1、P2、F1和F2群体进行了玉米粗缩病抗病性鉴定。并用168对引物进行了亲本间SSR-PCR扩增,其中48对引物在亲本间表现多态性。然后利用这些多态性引物检测F2的抗池和感池,只有6个SSR标记在F2的抗池和感池之间表现出多态性。这6个标记为:5号染色体上的Blng1237(Bin5.05-5.06)、umc1941(Bin5.06)、phi087(Bin5.06)、umc1155(Bin5.05)和9号染色体的phi065(Bin9.03)、umc1505(Bin9.07)。进一步利用这6个SSR标记检测了465个经抗病性鉴定的F2单株的基因型,其中Blng1237和phi087明显表现为偏分离。其余4个标记用于分析表型鉴定表现为抗的181个F2单株的基因型,并对每一标记的分离数值与其相应的孟德尔理论比例(共显性1∶2∶1)相比较,进行χ2适合度检验。结果表明,umc1155、umc1505这2个标记可能与抗性基因有关。The P1, P2, F1 and F2 of hybrids of Su 951 and 87-1 were identified with the method of group inoculation. 48 out of 168 random primers deteetced DNA potymorphism between Su 951 and 87-1 using the technique of SSR. And the SSR markers were used to detect the two F2 DNA pools. There were six markers which exhibited polymorphisms between the two F2 pools. The six markers were Blng1237 （ Bin5. 05 - 5. 06）, umc1941 （ BinS. 06）, phi087 （ Bin.5. 06）, unwl155 （ BinS. 05 ） from chromosome 5 and phiO65（Bing. 03） ,umclSOS（Bin9. 07） from chromosome 9. The six markers were further used to detect the F2 population consisting 465 investigated individuals, two markers exhibited segregation distortion. The chi-square test was employed to test each marker for the expected 1 ： 2：1 segregation ratio for codominant markers in the F2 population which showed resistance. The results indicated that urnc1155 and umc1505 were possibly related to resistance genes.

关 键 词：玉米 玉米粗缩病 分子标记 SSR BSA 

分 类 号：S513.035.3[农业科学—作物学]