陆地棉子叶离体培养诱导不定芽植株再生  

In Vitro Adventitious Bud Induced from Cotyledon and Plant Regeneration of Cotton(Gossypium hirsutum L.)

作　　者：许亚楠[1] 佘建明[1] 张保龙[1] 沈新莲[1] 倪万潮[1] 

机构地区：[1]江苏省农业科学院农业生物技术研究所,江苏南京210014

出　　处：《江苏农业学报》2008年第5期595-599,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省高新技术项目(BG2006307);江苏省农业科学院重点领域课题(005036410301)

摘　　要：以陆地棉种子无菌苗的子叶为外植体材料,研究不同苗龄的子叶、激素组合、硝酸银等对子叶诱导不定芽的影响。试验结果:15 h苗龄的子叶,在附加BAP 3.0 mg/L、IAA 1.0 mg/L、2ip 1.0 mg/L和AgNO35.0 mg/L的不定芽诱导培养基上,珂字棉312和泗棉3号的不定芽诱导频率分别为70%和60%左右。Effects of hormones, seedling ages and AgNO3 on induction of adventitious bud were studied in cotyledon of cotton （Gossypium hirsutum L. ）in vitro. The result showed that the cotyledon of 15 h seedlings was considered as the best explant for induction of adventitious bud. Cotyledon was cultured on the induction medium supplemented with BAP 3.0 mg/L, IAA 1.0 mg/L, 2ip 1.0 mg/L and AgNO35.0 mg/L, the frequencies of adventitious bud were about 70% and 60% in Coker 312 and Simian 3 respectively.

关 键 词：陆地棉 子叶 离体培养 不定芽 植株再生 

分 类 号：S562.035.2[农业科学—作物学]