红将军苹果离体叶片高频再生体系研究  

Establishment of adventitious shoot regeneration system from leaves of Red General Fuji apple cultivar(Malus domestica) in vitro

作　　者：姜中武[1,2] 苏佳明[1,2] 段小娜[2] 沙玉芬 于强[2] 宋来庆[2] 束怀瑞[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]烟台市农业科学研究院果树研究所,山东烟台265500

出　　处：《果树学报》2008年第6期797-800,F0003,共5页Journal of Fruit Science

基　　金：国家行业支撑项目(NYHYZX07-024);山东省科技攻关计划(2006GG3209016)

摘　　要：以中熟红富士品种红将军组培苗的叶片为试材,研究了离体叶片诱导再生不定芽过程中不同激素种类与组合、暗处理时间、取叶部位等因素对再生率的影响。结果表明,试管苗继代培养3～4代,当代培养25～30d,取顶部嫩叶3～4枚,剪除叶片边缘,将剪切的叶片中部以近轴面接触在最佳诱导分化培养基上:MS+TDZ1.0mg/L+IAA0.5mg/L+蔗糖30g/L,培养基pH值5.8,黑暗培养10d后,转入光照培养条件下,再生频率达100%,再生芽数平均4.6个。叶片再生不定芽分化后15～20d转接到苹果继代培养基MS+BA0.5mg/L+IBA0.1mg/L上,当不定芽长度约2cm时再转接到1/2MS+IBA0.5mg/L+NAA0.5mg/L+Ac0.5g/L+蔗糖2%的生根培养基上,光培养15d后,生根率可达86.0%。Experiment was carried out with the leaves of Red General Fuji apple cuhivar for studying the effects of hormones, dark treatment and the samples collected from the different part of the leaf on the regeneration rate so as to establish an adventitious shoot regeneration system in vitro. Results showed that the optimal explants were the 3 rd and 4th leaves from top of 25 d old cultures. The leaves were cut to three parts along main vein without edge , then the obverse surface of middle parts was placed on the optimal inducing medium, which was MS ＋ TDZ 1.0 mg/L ＋ IAA 0.5 mg/L ＋ sucrose 30 g/L, pH 5.8. After 10 d of dark treatment, all cultures were moved to the light-culture room, and the average number of regenerated buds was 4.6, with regeneration rate 100%. 15 to 20 d later from differentiation, the adventitious buds were transferred to subculture medium, namely MS ＋ BA 0.5 mg/L ＋ IBA 0.1 mg/L. When reaching 2 cm, they were transferred to rooting medium, which was 1/2MS ＋ IBA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ Ac 0.5 g/L ＋ sucrose 2%. After 15 d light-culture, rooting rate reached 86.0%.

关 键 词：富士苹果 红将军 离体叶片 再生体系 

分 类 号：S661.1[农业科学—果树学]