香蕉束顶病毒DNA组分的克隆及其重要特征序列分析(英文)  

Cloning of a DNA component of Banana bunchy top virus and analysis of its characteristic sequences

作　　者：黄永红[1] 梅眉 曾继吾[1] 周碧容[1] 吴元立[1] 夏瑞[1] 易干军[1] 

机构地区：[1]广东省农业科学院果树研究所,广州510640 [2]山东省胶州市农业局,山东青岛266300

出　　处：《果树学报》2008年第6期881-886,共6页Journal of Fruit Science

基　　金：广东省自然科学基金项目(06025368)

摘　　要：克隆得到1条新的香蕉束顶病毒(BBTV)6核苷酸全序列,对其进行序列分析发现,该序列含有2个主要的特征序列CR-SL(Stem-loop common regions)和CR-M(Major common regions)。BBTV6氨基酸进化树结果显示其分成2大类。将6种BBTV组分的序列进行比较发现,6种组分内核苷酸和氨基酸的最高相似性分别为96.10%和98.27%,6种组分间核苷酸和氨基酸最高相似性分别为70.78%和56.24%。6种BBTV组分的核苷酸及其氨基酸进化树将所有分离物按照组分类别分成6组,而6种BBTV组分的CR-M核苷酸序列进化树将所有的分离物按照地域分成2大类。对BBTV的6种组分的蛋白质进行分析,结果表明,各种组分蛋白质的二级结构和理化性质有明显的差异。A new full nucleotide sequence of Banana bunchy top virus （BBTV） component 6 was cloned and analyzed. The sequence contained two main characteristic sequences,the CR-SL （Stem-loop common regions） and the CR-M （Major common regions）. The phylogenetic tree constructed using the amino acid sequences of BBTV 6 suggested that BBTV contained two groups. The nucleotide and amino acid sequences of six BBTV components were aligned and compared, and the highest identities of the nucleotide and the amino acid sequences within each component were 96.10% and 98.27% respectively, while those among the six components were 70.78% and 56.24%, respectively. The phylogenetic trees based on full nucleotide sequences and amino acid sequences of the six BBTV components indicated six groups corresponding to each BBTV component. Based on CR-M nucleotide sequence, two groups corresponding to the places of origin could be divided. In addition, the secondary structures and some physicochemical properties of proteins of the six BBTV components were predicted. The result showed their significant differences.

关 键 词：香蕉束顶病毒 克隆 序列分析 特征序列 

分 类 号：S668.1[农业科学—果树学]