复合反硝化菌群培养及生化性能研究  

Culture of Complex Denitrification Sludge and Its Denitrification Properties

作　　者：陈英文[1] 陈徉[1] 沈树宝[1] 

机构地区：[1]南京工业大学国家生化工程技术研究中心,江苏南京210009

出　　处：《环境科学与技术》2008年第11期5-8,共4页Environmental Science & Technology

基　　金：国家"863"项目(2006AA02Z211);江苏省省级污染防治专项资金资助项目(2006-1-W-19);江苏省自然科学基金项目(BK2006181;BK2007188);江苏省环保科技项目(2007020)

摘　　要：根据反硝化过程中存在的问题,筛选到一株具有反硝化能力的菌株,鉴定为施氏假单胞菌（Pseudomonas stutzeri）,该菌株与生物活性炭污泥共培养,得到复合反硝化菌群,并对反硝化菌群的生化降解性能进行了研究。结果表明：该复合菌群污泥生物显微照片呈不规则颗粒状,SVI为38-55mL/g,VSS为2.0g/L,无丝状菌存在;具有明显的反硝化NO3^--N,NO2^--N能力,容积负荷可达0.9kgNO3^--N（/m^3·d）;单独NO3^--N,NO2^--N反硝化曲线基本相似,NO3^--N,NO2^--N共存基质反硝化过程存在底物竞争关系,但不发生NO2^--N抑制反硝化效应;在DO〈0.5mg/L条件下,反硝化效果良好;复合菌群可以在pH为9.3条件下发生良好反硝化反应而不受到pH抑制。With the purpose to solve the problems in the bio-denitlificafion process, a strain with denitrification ability was separated and identified as Pseudomonas stutzeri. Complex denitrification sludge was cultured by the coupling growth of the strain and bio-active carbon sludge, and the denitrification properties were studied. Results showed that the microphoto shape of the complex denitrification sludge was granular irregularly with no filamentous bacteria. SVI was 38-55mL/g and VSS was 2.0g/L. As for the different denitrification substrates such as NO3^--N and NO2^--N, the denitrification curves were similar. There was substrates competitive relationship between NO3^--N and NO2^--N when they coexisted in the same simulated wastewater, but no inhibitive effect occured by NO2^--N. When DO was below 0.5mg/L, the denitrification effect was good, and in the high pH of 9.3, denitrification could take place well. The volume loading could get to 0.9kgNO3^--N/（m^3·d）.

关 键 词：反硝化 施氏假单胞菌(Pseudomonas stutzeri) 复合菌群 NO3^--N NO2^--N 

分 类 号：X703.1[环境科学与工程—环境工程]