RP-HPLC法测定复方氨酚葡锌片中对乙酰氨基酚和盐酸二氧丙嗪的含量  

Determination of paracetamol and dioxopromethazine in compound paracetamol and zinc gluconate tablets by RP-HPLC

作　　者：王原瑞[1,2] 

机构地区：[1]黑龙江中医药大学,哈尔滨151000 [2]齐齐哈尔市药品检验所,黑龙江齐齐哈尔161006

出　　处：《齐齐哈尔大学学报（自然科学版）》2008年第6期81-83,共3页Journal of Qiqihar University(Natural Science Edition)

摘　　要：目的:采用高效液相法同时测定复方氨酚葡锌片中对乙酰氨基酚和盐酸二氧丙嗪的含量。方法:以C18化学键合硅胶为固定相,以水-乙腈-三乙胺(90﹕10﹕0.2)为流动相,检测波长为226 nm进行测定。结果:平均加样回收率对乙酰氨基酚为100.3%,RSD=0.71%(n=9);盐酸二氧丙嗪为98.79%,RSD=0.31%(n=9)。对乙酰氨基酚在20～200μg/ml范围内;盐酸二氧丙嗪在0.2～2μg/ml范围内,浓度与峰面积值呈良好的线性关系。结论:方法简便、快速、结果准确。Objective： A content determination of paracetamol and Dioxopromethazine in Compound Paracetamol and Zinc Gluconate tablets has been carried out by RP-HPLC Method： The C,s chemical bonded silica gel as a solid phase, water - acetontrile - triethylamine （90 ： 10 ： 0.2） as a mobile phase and UV detecting wavelength was at 226 nm. Result： The average recovery of the added paracetamolwas 100.3%, RSD=0.71 （n=9）and Dioxopromethazine was 98.79%, RSD=0.31（n=9）.There was a good linear relationship between the concentration and peak area in range of paracetamol 20 ～ 200μg/ml and Dioxopromethazine 0.2 ～ 2μg/ml.Conclusion：this method is accurate, rapit and simple.

关 键 词：RP-HPLC法 对乙酰氨基酚 盐酸二氧丙嗪 复方氨酚葡锌片 含量测定 

分 类 号：O657.72[理学—分析化学]