RSV RNA检测在中医药治疗小儿RSV肺炎的病原学评价中的应用  

Application of RSV and RNA Determination in Pathogenic Evaluation of Children's RSV Pneumonia Treated with Chinese Medicine

作　　者：汪小蓉[1] 高峰[1] 任现志[2] 汪受传[2] 杨学文[1] 

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]南京中医药大学第一临床医学院,江苏南京210046

出　　处：《南京中医药大学学报》2008年第6期373-375,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省中医药局资助课题(H05125);“十五”国家科技攻关计划项目(2004BA716B03)

摘　　要：目的对中医药治疗小儿呼吸道合胞病毒肺炎进行病原学疗效评价。方法中医药治疗小儿RSV肺炎的临床试验按照GCP规范进行,应用实时荧光PCR技术检测RSV RNA。结果对67例入选病例治疗4、71、0 d的鼻咽部分泌物进行RSV检测,结果显示:实验组和对照组RSV d 4d、7转阴比较,差异无统计学意义(P>0.05),d 10全部转阴。结论中医药治疗小儿呼吸道合胞病毒性肺炎,其病毒阴转率与对照组(西药组)没有差异。OBJECTIVE To carry out pathogenic evaluation on children＇s RSV pneumonia treated with Chinese medicine. METHOD The treatment of children＇s RSV pneumonia was based on GCP standard and real - time fluorescent PCR technique was used in the determination of RSV and RNA. RESULT RSV determination was carried out on the secretions from the nose and throat of 67 patients 4, 7 and 10 days after the treatment, with no significant differences from the control group （P〉 0.05）. All the patients showed negative results on the tenth day. CONCLUSION There is no significant difference in the negative rate in the treatment of children＇ s RSV pneumonia treated with Chinese medicine or western medicine.

关 键 词：呼吸道合胞病毒(RSV) 实时荧光PCR 病原学评价 病毒性肺炎 

分 类 号：R259.631[医药卫生—中西医结合]