适应菲胁迫的高效聚磷菌筛选及聚磷特性研究  

Screening of Two Phenanthrene-Utilizing and High-Effective Phosphorus-Accumulating Bacteria and Their Effects on Phosphorus-Accumulating Characteristics

作　　者：吴云[1,2] 范丙全[2] 隋新华[1] 龚明波[2] 

机构地区：[1]中国农业大学生物学院微生物学与免疫学系,北京100094 [2]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《环境科学》2008年第11期3172-3178,共7页Environmental Science

基　　金：国家重点基础研究发展规划(973)项目(2002CB410809)

摘　　要：采用平板法分离菌株、蓝斑筛选和聚磷培养液除磷能力验证3种方法相结合,从太湖底泥样品中分离到2株能够利用菲的高效聚磷菌Y11和Y4-2,经形态观察、生理生化和16S rDNA鉴定为不动杆菌属（Acinetobactersp.）.通过固体平板和液体培养的方法对2株不动杆菌的环境适应能力和聚磷、除磷能力进行了测定,结果显示,2菌株的生长温度范围均为10-35℃,菌株Y11的pH范围为6-9,菌株Y4-2的pH范围为6-8;高磷浓度对菌株Y11的生长没有抑制作用,但对菌株Y4-2的生长产生一定的抑制作用;Y11和Y4-2能在以菲为唯一碳源（50 mg/L）的无机盐平板上良好生长,对菲有一定的适应性.菌株Y11和Y4-2在30℃,170 r/min,1%接种量（体积分数,菌悬液D600=0.4）的条件下,2 mg/L磷浓度的聚磷培养液中最大聚磷率分别为96.13%和94.65%,培养液的磷浓度由2 mg/L分别降至0.08 mg/L和0.11 mg/L;5 mg/L磷浓度的聚磷培养液中最大聚磷率分别为95.94%和71.19%,培养液的磷浓度由5 mg/L分别降至0.20 mg/L和1.44 mg/L;8 mg/L磷浓度的聚磷培养液中最大聚磷率分别为71.24%和47.81%,培养液的磷浓度由8 mg/L分别降至2.30 mg/L和4.18 mg/L.使用2菌株处理云南滇池污水（磷含量为1.01 mg/L）,30℃,170 r/min,4%接种量（菌悬液D600=0.4）条件下,菌株Y11处理6 h后磷浓度由1.01 mg/L降至0.06 mg/L,菌株Y4-2处理48 h后磷浓度由1.01 mg/L降至0.06 mg/L.研究结果表明,菌株Y11和Y4-2对环境的适应性较强,均能高效、快速地降低聚磷培养液和云南滇池水体的磷浓度,不动杆菌Y11的除磷能力和环境适应性都大于菌株Y4-2,菌株Y11适用于南方和北方含磷较高的、菲污染的各种富营养化水体修复,菌株Y4-2更适用于pH8.0以下、低磷和菲污染的富营养化水体修复.In this study, two phenanthrene-utilizing and high-effective P-accumulating bacteria Y11 and Y4-2 were isolated from sludge samples of Taihu Lake using plate culture and blue-colored colonies methods. Strains Y11 and Y4-2 were identified as Acinetobacter sp. according to the results of morphology, physiology and the phylogenetical analyses of 16S rDNA sequences. The two strains showed high effective Paccumulating ability in plate and broth cultures. Y11 could grow well from 10℃ to 35℃ and pH from 6 to 9, Y4-2 could grow well from 10℃ to 35℃ and pH from 6 to 8. Phenanthrene could be used as sole carbon and energy sources. In our experiment, high phosphorus concentration in broth culture bad no negative effect on the growth of strain Y11, however the growth of strain Y4-2 was slightly affected. Under cultivation condition of 30℃, 170 r/min, 1% inoculation （D600 = 0.4）, the accumulation of phosphorus was various by strains and cultivation concentration of phosphorus as well： In 2 mg/L phosphorus concentration medium, the highest phosphorus-accumulation ratio of strains Y11 and Y4-2 were 96.13% and 94.65% , the phosphorus concentration decreased from 2 mg/L to 0.08 mg/L and 0.11 mg/L, respectively. In 5 mg/L phosphorus concentration medium, the highest phosphorus-accumulation ratio of strains Yll and Y4-2 were 95.94% and 71.19%, the phosphorus concentration decreased from 5 mg/L to 0.20 mg/L and 1.44 mg/L, respectively. In 8 mg/L phosphorus concentration medium, the highest phosphorus-accumulation ratio of strains Y11 and Y4-2 were 71.24% and 47.81%, the phosphorus concentration decreased from 8 mg/L to 2.30 mg/L and 4.18 mg/L, respectively. For the P removal of Yunnan Dianchi water sample containing 1.01 mg/L phosphorus, Y11 and Y4-2 were successful to decrease the phosphorus concentration from 1.01 mg/L to 0.06 mg/L in 6 b and 48 h, respectively. The results indicated that Acinetobacter sp.Yll and Y4-2 possess high ability of phosphorus removal and suitability to various water environments. Y11 stra

关 键 词：聚磷 不动杆菌 富营养化 污水处理 

分 类 号：X52[环境科学与工程—环境工程]