水稻脂氧合酶-3基因启动子的特性分析  

Characterization of the rice lipoxygenase-3 gene(Lox-3) promoter

作　　者：刘南南[1] 张文伟[1] 江玲[1] 沈文飚[1] 翟虎渠[2] 万建民[1,2] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室／江苏省植物基因工程技术研究中心,江苏南京210095 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《南京农业大学学报》2008年第4期1-7,共7页Journal of Nanjing Agricultural University

基　　金：国家973计划项目(2006CB101706,2004CB117204);国家863计划项目(2006AA10Z1A5,2006BAD13B01);江苏省农业种质资源基因库项目(sx(2007)g02);长江学者和创新团队发展计划项目(IRT0432)

摘　　要：从栽培水稻品种越光中克隆了水稻脂氧合酶-3基因(Lox-3)的启动子,长度为1343bp,其序列与日本晴对应的序列完全相同。生物信息学分析表明,该启动子序列中包含受信号诱导的元件(G-box)、温度响应元件(CCGAAA)和水分胁迫响应元件(CACATG)等顺式作用元件;进一步构建了Lox-3基因启动子与gus和gfp基因的融合表达载体并获得转基因植株,发现报告基因在转基因植株的根、茎、叶、花药、种胚及种皮中均有表达,在萌发种子的胚和胚芽中也有表达,但在胚乳中不表达。此外,经ABA、NaCl及人工陈化处理后对报告基因的表达进行了定量分析,发现这些非生物胁迫可能通过诱导启动子顺式元件致使报告基因表达活性增强。In this study,the Lox-3 promoter was cloned from the rice cultivar Koshihikari,and the length of the promoter is 1 343 bp,which absolutely matches the corresponding sequence of Nipponbare genome.Bioinformatics analysis indicated the promoter sequence contained a common signal-response cis-element（G-box）,elements responsive to temperature（CCGAAA）and to moisture stress（CACATG）.Furthermore,transgenic plants driven by this promoter was obtained.Reporter gene expression was detected in roots,stems,leaves,anthers,seed capsules and embryos of transgenic plants,as well as in the embryos and the plumule rather than in endosperm,of germinating transgenic seeds.A quantitative analysis revealed that the reporter gene was induced by exogenous application with ABA,NaCl and artificial aging treatments,and these effects were supposed to be mediated by the cis-elements in the corresponding promoter.

关 键 词：脂氧合酶-3基因 启动子 水稻 转基因植株 逆境胁迫 

分 类 号：Q943.2[生物学—植物学] S511[农业科学—作物学]