4种小肽衍生物的非水毛细管电泳分离研究  

Separation of Four Kinds of Oligopeptides Derivatives by Nonaqueous Capillary Electrophoresis

作　　者：王明清[1] 王延宝[1,2] 盛筱[1] 孙学军[1] 尤进茂[1,3] 

机构地区：[1]曲阜师范大学化学科学学院,山东曲阜273165 [2]济宁学院化学系,山东曲阜273165 [3]中国科学院西北高原生物研究所,青海西宁810001

出　　处：《分析测试学报》2008年第11期1241-1243,共3页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20075016)

摘　　要：以咔唑-9-乙基氯甲酸酯为柱前衍生试剂,采用非水毛细管电泳对衍生小肽进行分离。以甲醇和乙腈为溶剂,乙酸-乙酸铵为缓冲体系,采用235 nm二极管阵列检测。在甲醇与乙腈体积比为60∶40,乙酸铵浓度60 mmol/L,乙酸浓度0.8 mol/L,柱温20℃,分离电压30 kV条件下,实现了4种小肽衍生物的基线分离。A simple nonaqueous capillary electrophoresis method was proposed for rapid separation of derivatized oligopeptides using 9-（2-carbazole ） ethyl chloroformate （CEOC） as the pre-column derivatization reagent. A mixture of methanol and aeetonitrile （60 ： 40, by volume ） was used as the background electrolyte solvent, 0. 8 mol/L acetic acid - 60 mmol/L ammonium acetate was used as the buffer. A satisfactory baseline separation of four derivatized oligopeptides was obtained at detection wavelength of 235 nm, column temperature of 20℃ and applied voltage of 30 kV.

关 键 词：小肽 非水毛细管电泳 咔唑-9-乙基氯甲酸酯(CEOC) 

分 类 号：O657.8[理学—分析化学] O629.72[理学—化学]