高盐胁迫下的草原龙胆基因表达分析  

Gene Expression of Eustoma Grandflorum under High Salinity Stress

作　　者：王继刚[1] 张坤[2] 李葵花[1] 冯国军[3] 李玉花[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]西南林学院学报编辑部,云南昆明650224 [3]哈尔滨市农业科学院,黑龙江哈尔滨150070

出　　处：《北方园艺》2008年第11期139-142,共4页Northern Horticulture

基　　金：“948”资助项目(2005-4-35);黑龙江省“十一五”公关课题资助项目(GB06B112-5)

摘　　要：为了更好的理解植物对盐胁迫的反应的分子机理,结合差减抑制杂交技术和基因芯片的方法,研究了草原龙胆对于高盐胁迫响应重要性和新基因。先利用草原龙胆50#叶片进行盐处理和非盐处理,并以此构建抑制差减文库。从此文库中选取部分克隆构建了草原龙胆基因芯片,进行了芯片分析,研究了高盐处理对非处理条件下的基因表达变化。在3 h的高盐胁迫下,基因芯片分析显示有36个单一基因有差异表达,大多数基因未见报道。这些基因的存在表明盐胁迫导致了草原龙胆复杂的胁迫响应。To understand the molecular basis of plant responses to salt stress better, suppression subtractive hybridization （SSH） and microarray approaches were combined to identify the potential important or novel genes of Eustoma grandi florurn involved responses to severe salt stress. First, SSH libraries were constructed for the leave tissue of cultivated Eustomagrandiflorum 50^# to compare salt treatment and non-treatment plants. Then a subset of clones from these SSH libraries were used to construct a Eustoma grandiflorum cDNA array and microarray analysis was carried out to verify the gene expression changes of this set of clones upon a high concentration of salt treatment compared to the corre- sponding non--treatment controls. A total of 36 non-redundant genes that were differentially expressed upon 3 h of severe salt stress were identified from microarray analysis; most of these genes have not previously been reported. The di- versity of these genes indicated that salt stress resulted in a complex response in Eustoma grandiflorun.

关 键 词：草原龙胆 芯片 抑制差减杂交 盐胁迫 

分 类 号：S567.239[农业科学—中草药栽培]