西伯利亚百合子房的组织培养及离体快繁研究  被引量:7

In Vitro Ovary Culture and Rapid Propagation of Siberia

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作  者:申玉华[1] 段永平[1] 唐立红[1] 黄园[1] 李超[1] 

机构地区:[1]赤峰学院生命科学系,内蒙古赤峰024000

出  处:《北方园艺》2008年第11期151-152,共2页Northern Horticulture

摘  要:以子房为外植体,对西伯利亚百合离体快繁进行研究。结果表明:试验浓度范围内适合不定芽诱导的培养基以MS+6-BA 1.0 mg/L+NAA 1.5 mg/L为佳,芽增殖最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,适合生根的培养基为MS0,生根率达100%,且根系发达,长势粗壮,练苗3-5 d后移栽,长势良好,移栽成活率达90%以上。Rapid propagation of Siberia was preliminary studied using ovaries for in vitro culture. Result showed that the optimum mediums for shoot induction, shoot proliferation and rooting were MS+6-BA 1.0 mg/L+NAA 1.5 mg/L, MS+6-BA 1.0 mg/L+NAA 0.2 rag/ L and MS0 respectively. Rooting rate could reach 100% and its root system was well developed, after acclimatization for 3 - 5 days, the bulblets were transplanted and grew well, survival rate after transplanting was above 90%.

关 键 词:西伯利亚百合 组织培养 子房 

分 类 号:S682.29[农业科学—观赏园艺] S603.6[农业科学—园艺学]

 

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