磺胺二甲嘧啶抗体的制备及酶联免疫分析方法的建立  

Preparation of Anti-sulfamethazine Antibody and Establishment of Enzyme-Linked Immunosorbent Assay for Sulfamethazine

作　　者：李子颖[1] 刘一兵[1] 袁志刚[1] 许文革[1] 冯婷婷[1] 郑婷[1] 

机构地区：[1]中国原子能科学研究院同位素研究所,北京102413

出　　处：《原子能科学技术》2008年第B09期63-67,共5页Atomic Energy Science and Technology

摘　　要：应用人工合成免疫原免疫动物、杂交瘤融合技术制备出抗磺胺二甲嘧啶的单抗及多抗。抗体亲和常数均大于1.0×108L/mol。利用多抗2510建立酶联免疫分析方法。该方法测量范围为0.1^30 ng/mL,灵敏度为0.08 ng/mL,批内变异〈15%,批间变异〈21%。不同样品回收率在80.5%^137.8%之间,稀释实验测定值与稀释度呈线性相关,相关系数为0.997。Polyclonal antibodies （PcAbs） and monoclonal antibodies （McAbs） against sulfamethazine （SM2） were obtained. The association constants （Ka） of the antibodies are all higher than 10^8 L/mol. The enzyme-linked immunosorbent assay （ELISA） for the measurement of SM2 was set up by using PcAb-2510. The assay range for this method is 0. 1-30 ng/mL and the sensitivity is 0.08 ng/mL. The intra-assay and inter-assay coefficients of variation （CV） vary from 12.8%-14. 1% and 14.0%-20.5%, respectively. The recovery of the analyte in different samples is from 80. 5% to 137.8% and the experimental correlation coefficient of dilution is found to be 0. 997.

关 键 词：酶联免疫分析方法 磺胺二甲嘧啶 抗体 

分 类 号：R978.2[医药卫生—药品]