麻杏石甘汤对肥大细胞脱颗粒的影响  

Effect of Maxing Shigan Decoction on degranulation of mast cell

作　　者：黄丰[1] 童晓云[2] 张荣华[1] 蔡宇[1] 

机构地区：[1]暨南大学药学院,广东广州510632 [2]广州中医药大学第一附属医院,广东广州510405

出　　处：《中成药》2008年第11期1582-1584,共3页Chinese Traditional Patent Medicine

基　　金：广东省中医药局建设中医药强省科研课题(No.2007351)

摘　　要：目的:探讨麻杏石甘汤(麻黄、杏仁、甘草、石膏)抗Ⅰ型变态反应的作用机制。方法:通过大鼠被动皮肤过敏反应(PCA)实验,用比色测定法检测麻杏石甘汤在体内对Ⅰ型变态反应的影响;体外实验先制备麻杏石甘汤的含药血清,然后将其加入致敏的RBL-2H3细胞中,观察麻杏石甘汤对RBL-2H3细胞脱颗粒及其他炎性物质的影响。结果:麻杏石甘汤药物血清能明显抑制RBL-2H3细胞脱颗粒,并能抑制RBL-2H3细胞释放组胺、肿瘤坏死因子α及白细胞因子4。结论:麻杏石甘汤的抗过敏作用与抑制肥大细胞的脱颗粒及炎性物质释放有关。AIM ： To investigate the mechanism of Maxing Shigan Decoction （ Herba ephedrae, Semen armeniacae amarum, Radix er Rhizoma glycyrrhizae, Gypsum fibrosum） against type I hypersenstivity reaction. METHODS： The experiment of passive cutaneous anaphylaxis （PCA） was used to determine the effect of Maxing Shigan Decoction on degranulation of mast cells in vivo. For in vitro study, the drug serum of Maxing Shigan Decoction was added to the culture medium of sensitized RBL-2H3 cells. The antigen-induced release of degranulation was measured by enzymatic assay, histamine by enzyme immunoassay （EIA） and cytokines by enzyme-linked immunosorbent assay （ELISA）. RESULTS： The results showed that treatment with drug serum of Maxing Shigan Decoction was followed by a decrease in PCA of rats and in degranulation, histamine, TNF-α and IL-4 from RBL-2H3 cells induced by antigen. CONCLUSION： Maxing Shigan Decoction may suppress the type I hypersensitivity reaction by inhibiting the action of mast cells.

关 键 词：麻杏石甘汤 被动皮肤过敏反应 肥大细胞 脱颗粒 

分 类 号：R285.6[医药卫生—中药学]