桫椤叶总黄酮正交提取工艺和大孔树脂吸附分离特性的研究  

Extraction of Alsophila spinulosa(Hook.) Tryon flavonoids by orthogonal experiment and separation by macroporous resin

作　　者：陈封政[1] 李书华[1] 向清祥[1] 

机构地区：[1]乐山师范学院化学与生命科学系,四川乐山614004

出　　处：《中成药》2008年第11期1600-1604,共5页Chinese Traditional Patent Medicine

基　　金：四川省科技厅应用基础研究资助项目(05JY029);四川省教育厅自然科学研究基金资助项目(2005A165)

摘　　要：目的:研究桫椤叶总黄酮的提取工艺和大孔树脂吸附纯化的工艺参数。方法:采用单因素和正交实验法,以总黄酮的提取率为考察指标和采用树脂静态、动态吸附脱附实验法,以总黄酮吸附率、解吸率等为考察指标。结果:桫椤叶总黄酮最佳提取工艺为:提取温度为80°C,提取时间为1 h,料液比为1∶12,乙醇浓度为60%,提取2次;树脂D104对桫椤叶总黄酮吸附分离的最佳工艺:上柱液质量浓度1.36 mg/mL,上柱流速2 BV/h,溶液处理量为5 BV/次,洗脱溶剂为70%的乙醇,脱附流速为3 BV/h,脱附剂用量为2 BV/次。结论:该提取精制路线达到富集有效成分的目的。AIM： To study the optimum extraction process of total flavonoids in Alsophila spinulosa （Hook.） Tryon and technological parameter of macroporous resin. METHODS ： The optimum parameters were chosen by ratio of total flavonoids as the assessment index such as static and dynamic adsorption of macroporous resin. RESULTS ： The optimum extracting process conditions were as follows ： solvent was ethanol, ratio of solid-liquid was 1 ： 12, extraction time was 1 h, temperature was at 80 ℃. The optimum technological conditions of the D104 were： the solution concentract at 1.36 mg/mL, the current velocity of adsorption 2 BV/h, the amount of saturated adsorption at 5 BV, the eluting reagent 70% ethanol, the eluting velocity 3 BV/h, eluent volume at 2 BV. CONCLUSION： The process can separate and concentrate the total flavoionds of Alsophila spinulosa （Hook.） tryon.

关 键 词：桫椤 总黄酮 正交实验 D104大孔树脂 

分 类 号：R284.2[医药卫生—中药学]