木霉菌素产生菌的诱变育种  

Study on Mutation Breeding of Trichodermin Producing Strain

作　　者：石一珺[1] 申屠旭萍[2] 俞晓平[3] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310034 [2]中国计量学院生命科学学院,浙江杭州310018 [3]浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《安徽农业科学》2008年第29期12806-12807,12812,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省自然科学基金项目(Y307128);浙江省重大招标项目(2004C12015;2006C12032)

摘　　要：[目的]筛选高产木霉菌素菌株,提高木霉菌素产量。[方法]以从枸骨中分离到的产木霉菌素的内生真菌———哈茨木霉为出发菌株,进行紫外线二次复合诱变处理,将筛选出的突变菌株连续转接5代,测定其遗传稳定性。[结果]随着照射时间的延长,哈茨木霉的致死率增大,选取致死率为88.1%的紫外线照射45 s作为最适处理剂量进行诱变育种。经过2次诱变的菌株的产孢时间提前,长出的菌落更为致密。经过初筛和复筛,最终获得1株高产突变株UV-5-3,其产抗生素的水平最高,为164.75μg/m l,比初次诱变筛选获得的突变株UV-3-1提高了56.77%,是出发菌株的2.3倍。传代试验表明,突变株UV-5-3的高产性能遗传特性稳定。[结论]利用紫外线二次复合诱变处理哈茨木霉可以获得高产木霉菌素菌株。[ Objective] The aim was to screen the high yield trichodermin strain and increase the yield of trichodermin. [ Method] With Trichoderma harzianum, an endophytie fungi producing triehodermin separated from Llex cornuta, as the original strain for the treatment of ultraviolet radiation compound mutation for two times, the 5 generations of the screened mutation strains were transferred continuously and its genetic stability was detected. [ Result] The lethality rate of T. harzianum was increased with the prolonging of the irradiation time. The ultraviolet ir- radiation of 45 s with the lethality rate of 88.1% was selected as the optimum mutation dose for mutation breeding. The sporulation time of the strain through twice mutation was advanced and the growing strain was dense. A high yield mutation strain UV-5-3 was obtained at last through the primary screening and the secondary screening and its antibiotic level was the highest, being 164.75 μg/ml, which was 56.77% higher than that of UV-3-1 mutation strain obtained through the primary screening, and the 2.3 times of the original strain. The passage experiment showed that the high yield preference of the mutation strain UV-5-3 had stable genetic characteristie. [ Conclusion] The high yield trichodermin strain could be obtained by treating T. harzicmum through ultraviolet radiation compound mutation treatment for two times.

关 键 词：木霉菌素 哈茨木霉 紫外诱变 筛选 

分 类 号：Q933[生物学—微生物学]