SarA influences the sporulation and secondary metabolism in Streptomyces coelicolor M145  

作　　者：Xijun Ou Bo Zhang Lin Zhang Kai Dong Chun Liu Guoping Zhao Xiaoming Ding 

机构地区：[1]State Key Laboratory of Genetic Engineering, Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China [2]Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Centre at Shanghai, Shanghai 201203, China [3]Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

出　　处：《Acta Biochimica et Biophysica Sinica》2008年第10期877-882,共6页生物化学与生物物理学报（英文版）

基　　金：This work was supported by a grant from the National Natural Science Foundation of China （No. 30600009）

摘　　要：The filamentous bacteria Streptomyces exhibit a complex life cycle involving morphological differentiation and secondary metabolism. A putative membrane protein gene sarA （sco4069）, sporulation and antibiotic production related gene A, was partially characterized in Streptomyces coelicolor M145. The gene product had no characterized functional domains and was highly conserved in Streptomyces. Compared with the wild-type M145, the sarA mutant accelerated sporulation and dramatically decreased the production of actinorhodin and undecylprodigiosin. Reverse transcription-polymerase chain reaction analysis showed that SarA influenced antibiotic production by controlling the abundance ofactll-orf4 and redZ messenger RNA.The filamentous bacteria Streptomyces exhibit a complex life cycle involving morphological differentiation and secondary metabolism. A putative membrane protein gene sarA （sco4069）, sporulation and antibiotic production related gene A, was partially characterized in Streptomyces coelicolor M145. The gene product had no characterized functional domains and was highly conserved in Streptomyces. Compared with the wild-type M145, the sarA mutant accelerated sporulation and dramatically decreased the production of actinorhodin and undecylprodigiosin. Reverse transcription-polymerase chain reaction analysis showed that SarA influenced antibiotic production by controlling the abundance ofactll-orf4 and redZ messenger RNA.

关 键 词：Streptomyces coelicolor SPORULATION antibiotic production SARA 

分 类 号：Q939[生物学—微生物学]