Identification and characterization of genotype A and D recombinant hepatitis B virus from Indian chronic HBV isolates  

作　　者：Ranjit Chauhan Syed Naqui Kazim Manoj Kumar Jayashree Bhattacharjee Narayanasamy Krishnamoorthy Shiv Kumar Sarin 

机构地区：[1]Department of Gastroenterology and Advanced Center for Liver Diseases,G.B.Pant Hospital [2]Department of Biochemistry,Lady Hardinge Medical College [3]Centre for Interdisciplinary Research in Basic Sciences [4]The Centre for Genomic Application

出　　处：《World Journal of Gastroenterology》2008年第40期6228-6236,共9页世界胃肠病学杂志（英文版）

基　　金：Indian Council of Medical Research-Advanced Center for Liver Diseases Project (ICMR-ACLD)

摘　　要：AIM： To confirm the presence of recombination, fulllength hepatitis B virus （HBV） from chronic patients was sequenced and analyzed. METHODS： Full-length HBV genomes from 12 patients were amplified and sequenced in an automated sequencer. Phylogenetic analysis was carried out on full-length, Core and preS2/Surface regions using MEGA software. SimPIot Boot Scanning and amino acid sequence analysis were performed for confirmation of recombination. RESULTS： Eight patients were infected with genotype D strain; one patient with genotype A and three patients had genotype A and D recombination; two of them had cirrhosis and one had hepatocellular carcinoma. Phylogenetic analysis of core and preS2/surface regions separately showed evidence of genotype A and D recombination. The breakpoints of recombination were found to be at the start of preS2 and at the endof surface coding regions. CONCLUSION： We identified and characterized recombinant A and D genotype HBV in hepatitis B surface antigen （HBsAg）-positive patients.AIM: To confirm the presence of recombination,full-length hepatitis B virus (HBV) from chronic patients was sequenced and analyzed.METHODS: Full-length HBV genomes from 12 patients were amplified and sequenced in an automated sequencer.Phylogenetic analysis was carried out on full-length,Core and preS2/Surface regions using MEGA software.SimPlot Boot Scanning and amino acid sequence analysis were performed for confirmation of recombination.RESULTS: Eight patients were infected with genotype D strain; one patient with genotype A and three patients had genotype A and D recombination; two of them had cirrhosis and one had hepatocellular carcinoma.Phylogenetic analysis of core and preS2/surface regions separately showed evidence of genotype A and D recombination.The breakpoints of recombination were found to be at the start of preS2 and at the end of surface coding regions.CONCLUSION: We identified and characterized recombinant A and D genotype HBV in hepatitis B surface antigen (HBsAg)-positive patients.

关 键 词：Hepatitis B virus GENOTYPE Variation Evolution Recombination 

分 类 号：R512.62[医药卫生—内科学]