Galanin(甘丙肽)及其受体在成年小鼠脑内神经新生部位的表达及对神经干细胞分化的作用  

EXPRESSION OF GALANIN AND GALANIN RECEPTORS IN NEUROGENESIS REGIONS OF ADULT MOUSE BRAIN AND EFFECT OF GALANIN ON THE NEURAL STEM CELL′S DIFFERENTIATION

作　　者：马骥[1] 单良[1] 于玲莉[1] 袁崇刚[1] 

机构地区：[1]华东师范大学生命科学学院生理学研究室,上海200062

出　　处：《分子细胞生物学报》2008年第5期359-366,共8页Journal of Molecular Cell Biology

摘　　要：Galanin(甘丙肽)是一种在中枢神经系统中广泛分布的神经肽,功能涉及摄食、睡眠和觉醒、疼痛、认知和生殖等各方面。我们在成年小鼠脑的神经细胞新生部位如SVZ,DG和RMS发现有galanin及其受体的mRNA表达,同时在SVZ来源的神经干细胞中也检测到有galanin及其受体的表达。细胞实验中,在分化后特定时间段GALKO小鼠来源的神经干细胞产生神经突的细胞比例及神经突的长度明显小于正常小鼠来源的神经干细胞,而加入galanin或受体激动剂GAL2-11后,该神经干细胞则在产生神经突的细胞比例及神经突的长度都明显上升。受体拮抗剂M35的添加可减弱galanin或GAL2-11所产生的作用。这些结果表明galanin及其受体与神经干细胞的分化及神经突的生长有着密切的联系,并可能参与了神经系统的发育。The neuropeptid galanin is widely expressed in the central nervous system and has a diverse range of physiological effects including food intaking, arousal/sleep, nociception and reproduction. In this study, expression of galanin and galanin receptors （GalR1 and GalR2） mRNA were identified not only in the neurogenisis regions including subventrieular zone （SVZ）, rostral migratory stream （RMS） and dentate gyms （DG） of adult mice but also in the SVZ-derived neural stem cell （NSC） culture. Here, we also showed that the addition of galanin and GalR2-specific agonist Gal2-11 to wild-type or GALKO NSCs under differentiation condition significantly promote the neuritogenesis and increase the length of neurites on the βⅢ-tubulin positive cells. This effect could be reduced by treatment of the galanin antagonist M35. These results indicate that galanin and its receptors might regulate neurite extension in differentiating neural stem cells and even participate in the development of the nervous system.

关 键 词：GALANIN galanin受体 mRNA表达 神经干细胞 神经突生长 

分 类 号：Q42[生物学—神经生物学]