处理方式不同的颗粒细胞和卵泡液对牛卵母细胞体外受精后卵裂率和囊胚率的影响  

EFFECTS OF GRANULOSA CELLS TREATMENTS AND FOLLICULAR FLUID ON CLEAVAGE RATE AND BLASTOCYST RATE OF BOVINE OOCYTE AFTER IN VITRO FERTILIZATION AND CULTURE

作　　者：邓雯[1] 禹学礼[1] 庞有志[1] 昝林森[2] 

机构地区：[1]河南科技大学动物科技学院,洛阳471003 [2]西北农林科技大学动物科技学院,杨凌712100

出　　处：《分子细胞生物学报》2008年第5期393-402,共10页Journal of Molecular Cell Biology

基　　金：国家"十五"攻关计划 (2002BA514A-2-2);国家科技支撑计划 (2006BAD04A);洛阳市科技攻关重大项目(030218);河南科技大学人才科研基金项目(05-087)。

摘　　要：将牛的卵母细胞置于添加有不同预处理颗粒细胞及含有卵泡液的培养液或不含有卵泡液的培养液中进行体外成熟、受精及胚胎发育培养, 研究了颗粒细胞、卵泡液及颗粒细胞与卵泡液交互作用对牛卵母细胞成熟、受精后卵裂率、囊胚率的影响。 2178 枚卵母细胞体外成熟受精后胚胎发育的对比观察结果表明: 颗粒细胞、卵泡液及颗粒细胞与卵泡液交互作用对卵母细胞成熟受精后胚胎的卵裂具有显著影响 (P<0.05); 颗粒细胞对囊胚的发育有显著影响 (P<0.05); 卵泡液及颗粒细胞与卵泡液交互作用对囊胚的发育无显著影响 (P>0.05)。不同因素对卵裂率、囊胚率的影响表现为: 颗粒细胞因素﹥培养液因素﹥培养液×颗粒细胞交互作用。结论: TCM199 培养液中添加卵泡液和单层颗粒细胞组成的培养系统用于牛卵母细胞体外成熟及胚胎发育的效果较好。共培养体系中的单层颗粒细胞用经酶消化分散处理后在培养箱中孵育 10min 的颗粒细胞替代时, 胚胎的发育效果并不受影响。Experiments were conducted to study the effects of granulosa cell （GC）, follicular fluid and their interaction on cleavage rate and blastocyst rate of Bovine oocytes following in vitro maturation （IVM）, in vitro fertilization （IVF） and in vitro culture （IVC）. A total of 2178 oocytes were used for studies on maturation, fertilization and embryo development. The cleavage rate was affected significantly （P〈0.05） by GC, follicular fluid and the interaction of GC and follicular fluid. The blastocyst rate was affected significantly （P〈0.05） by GC, the blastocyst rate wasn＇t affected significantly by GC, follicular fluid and the interaction of GC and follicular fluid （P〉0.05）. Effects of granulosa cell and follicular fluid and their interaction on the cleavage rate and the blastocyst rate in embryo cultured sort order was GC 〉 follicular fluid 〉 interaction of follicular fluid and GC. It was concluded that follicular fluid and granulosa cell monolayer （GCM） adding in TCM199 better support maturation of oocytes and development of embryos in Bovine. There were no differences in the cleavage rate and blastocyst rate, between the groups granulosa cell monolayer and incubating granulosa cell （which were incubated 10min in incubator before oocytes being transferred） on co-culture system. Granulosa cell monolayer could be replaced by incubating granulosa cell on coculture system on Bovine oocytes IVM, IVF and IVC.

关 键 词：牛卵母细胞 颗粒细胞 卵泡液 体外受精 卵裂率 囊胚率 

分 类 号：Q813[生物学—生物工程] S814[农业科学—畜牧学]