取向聚乙醇酸聚乳酸共聚物支架的制备及初步实验观察  

Fabrication and characteristics of oriental poly （ lactic-co-glycolic acid） scaffold： a preliminary study

作　　者：余方圆[1] 卢世璧[1] 王身国[1] 赵斌[1] 贝建中[1] 许文静[1] 袁玫[1] 

机构地区：[1]解放军总医院骨科研究所,北京100853

出　　处：《中华外科杂志》2008年第21期1662-1664,共3页Chinese Journal of Surgery

基　　金：国家863计划资助项目（2002AA205021）

摘　　要：目的研制聚乙醇酸聚乳酸共聚物（PLGA）材料的纵向取向支架，观察体外生物相容性、细胞在支架内分布等特性，探讨其作为关节软骨组织工程支架的可能性。方法相分离法制备纵向取向PLGA支架，等离子改性、胶原包埋处理，体外接种关节软骨细胞，在环境扫描电镜下观察细胞生长、分布情况。结果研制的PLGA取向支架生物相容性明显提高，细胞沿纵向管道垂直排列，细胞分布均匀，单侧加载细胞进入深度可达2．5mm。结论取向支架能明显改善细胞在载体内的分布，细胞纵向排列接近关节软骨细胞排列方式。Objective To explore the method of fabricating oriental scaffolds and investigate the biocompatibility of the scaffolds as well as cells distribution within the scaffolds in vitro. Methods The oriental poly （lactic-co-glycolic acid ） （PLGA） scaffolds were fabricated with modified emulsion-phase separation method. The scaffolds were treated with plasma and then anchored with collagen Ⅰ. Artiticular chondrocytes were loaded into the scaffolds. The growth status and distributing characteristic of the cells were investigated by environmental scanning electron microscope. Results The scaffold was well compatible with the articular chondrocytes. The cells could reach to 2. 5 mm depth with unilateral loading. The cells distributed evenly in the scaffold and lined along the inner pipes. Conclusions The oriental scaffold fabricated could significantly promote the distributing characteristics of the chondrocytes. The vertical alignment of the chondrocytes within the scaffold is closely simular to that of artilage cartilage.

关 键 词：软骨 关节 组织工程 支架 

分 类 号：R686[医药卫生—骨科学]