淫羊藿甙对小鼠T淋巴细胞体外活化和增殖的影响  

Effects of icariin on activation and proliferation of murine T lymphocytes in vitro

作　　者：滕菲[1] 曾耀英[1] 黄秀艳[1] 杨志[1] 姚满林[1] 宋兵[1] 李林[1] 

机构地区：[1]暨南大学组织移植与免疫中心,广州510632

出　　处：《免疫学杂志》2008年第6期613-617,共5页Immunological Journal

基　　金：国家"973"重点基础研究项目(2006CB504201和2004CB720100);广东省基金项目(2006B36030016);广州市科技局科技攻关重点项目(2006Z-E0091)资助

摘　　要：目的研究淫羊藿甙(ICA)对刀豆蛋白A(ConA)刺激的小鼠T淋巴细胞体外早期活化和增殖的影响。方法以MTT法检测T细胞药物毒性;利用流式细胞术(FCM)结合双色荧光抗体染色技术检测早期活化标志CD69分子的表达;运用流式细胞术结合活体染料羧基荧光素乙酰乙酸(CFDA-SE)染色技术和荧光抗体染色技术检测T细胞增殖。结果ICA在终浓度0.3、1.5、3.0μmol·L-1时对于CD69的表达以及淋巴细胞48和72h增殖,均有明显的抑制作用(P<0.01)。结论ICA能抑制ConA刺激的小鼠T淋巴细胞的早期活化和增殖,并呈剂量依赖性,即在最高浓度3.0μmol·L-1时抑制率最强。Objective To study the effects of icariin （ICA） on the early activation and proliferation of routine T lymphocytes stimulated by concanavalin A （Con A） in vitro. Methods The chug toxicity of ICA on T lymphocytes was measured by MTT. Before stimulation with Con A （the final concentration was 5 mg·L^-1 ）, T ceils were treated with different concentrations of ieafiin for 4 h. The CD69 expression, the marker of early activation of T lymphoeytes, was measured by flow cytometry （FCM） combined with two-colour immunofluorescent staining of cell surface antigen. The proliferation of T lymphocytes was measured by flow cytometly combined with carboxyl fluorescin diaeetate succinimidyl ester （CFDA-SE） staining and immunofluorescent staining. Results ICA of 0. 3, 1.5, and 3.0 μmol·L^-1 inhibited the expression of CD69 （ P 〈 0. 01 ） and the proliferation of T cells （ P 〈 0.01 ）. Conclusion ICA significantly inhibits Con A-induced early activation and proliferation of mouse T lymphocytes in vitro, with a maximal inhibition in 3.0 μmol·L^-1 .

关 键 词：淫羊藿甙 T淋巴细胞 活化 增殖 流式细胞术 

分 类 号：R285.5[医药卫生—中药学]