红车轴草提取物对小鼠淋巴细胞[Ca^(2+)]_i及腹腔巨噬细胞NO分泌和吞噬的影响  

Effects of T. Leguminosae extract on [Ca^(2+)]_i of lymphocytes and the NO secretion and phagocytosis of macrophages in mouse

作　　者：杨志[1] 黄秀艳[1] 曾耀英[1] 滕菲[1] 李林[1] 宋兵[1] 姚满林[1] 

机构地区：[1]暨南大学组织移植与免疫实验中心,广州510632

出　　处：《免疫学杂志》2008年第6期638-640,643,共4页Immunological Journal

基　　金：“973”国家重大基础研究项目(2006CB504201,2004CB7-20100);广东省基金项目(2006B36030016);广州市科技局科技攻关重点项目(2006Z-E0091)资助

摘　　要：目的探讨红车轴草提取物(Trifoliumpratense Leguminosae extract,TLE)体外对小鼠淋巴细胞[Ca2+]i及腹腔巨噬细胞NO分泌和吞噬微球的影响。方法无菌条件下制备小鼠淋巴细胞悬液及小鼠腹腔巨噬细胞悬液;MTT法检测药物对细胞悬液的毒性情况;Fluo-4/AM染色结合流式细胞术分析TLE对小鼠淋巴细胞[Ca2+]i的影响;Griess反应系统检测TLE对脂多糖(LPS)刺激的小鼠腹腔巨噬细胞NO分泌的影响;1μm与2μm直径的荧光微球结合流式细胞术分析TLE对小鼠腹腔巨噬细胞吞噬作用的影响。结果终质量浓度为20、40mg/L的TLE对细胞的毒性小;TLE促进了淋巴细胞的Ca2+内流;TLE抑制了巨噬细胞的NO分泌与吞噬作用,与非TLE组比较P<0.01。结论对淋巴细胞[Ca2+]i及巨噬细胞的NO分泌和吞噬的作用可能是TLE调节小鼠免疫系统的途径。Objective To study the effects of Trifoliumpratense Leguminosae extract （TLE） on [ Ca^2 ＋ ]i of lymphocytes and the NO secretion and phagocytosis of macrophages from mouse in vitro. Methods Mouse lymphocytes suspension and macrophages suspension were prepared under germ-free condition; the cytotoxic effect of TLE on cell suspension was estimated by MTr test; Fluo-4/AM staining plus flow cytometry were used to detect [ Ca^2 ＋ ] i of lymphocytes from mouse; the effects of TLE on NO secretion of macrophages from mouse was detected by Griess reagent system; fluorescence microbeards （diameter： 1 tan and 2 pan） plus flow cytometry were used to analyze phagocytosis of macrophages of mouse. Results At the mass concentrations of 20 and 40 mg/L, TLE has little cytotoxic effect on cell suspension. TLE could promote the Ca^2＋ inflow of lymphocytes and inhibit the NO secretion and phagocytosis of macrophages of mouse （ P 〈 0.01 ）. Conclusion Regulating effects of TLE on the immunology system of mouse maybe due to the regulation of the [ Ca^2＋ ] i of lymphocytes and the NO secretion and phagocytosis of macrophages in mouse.

关 键 词：红车轴草提取物 淋巴细胞 钙离子 一氧化氮 巨噬细胞 吞噬 

分 类 号：R285.5[医药卫生—中药学]