低氧对卵巢癌SKOV3细胞乙酰肝素酶表达及侵袭力的影响  

Effects of hypoxia on the expression of heparanase and invasiveness of SKOV3 ovarian cancer cell line

作　　者：顾双[1] 袁彩霞[1] 张淑红[2] 张向宁[1] 

机构地区：[1]山东大学齐鲁医院妇产科,山东济南250012 [2]解放军456医院妇产科,山东济南250031

出　　处：《中国病理生理杂志》2008年第11期2175-2178,共4页Chinese Journal of Pathophysiology

摘　　要：目的:探讨低氧对卵巢癌细胞乙酰肝素酶(HPA)表达及侵袭力的影响。方法:将SKOV3细胞置于常氧(37℃,5%CO2,21%O2)和低氧(37℃,5%CO2,1%O2)12、24、36h条件下培养,采用RT-PCR及Western blotting方法对HPA表达进行检测,采用基质凝胶侵袭实验测定各组细胞侵袭力。结果:与常氧组比较,SKOV3细胞在低氧12、24、36hHPAmRNA表达增高,以12h增加显著,蛋白表达则依次增加,各组比较差异显著(P<0.01),而低氧各组间比较,HPA表达量亦不同(P<0.05);常氧组侵袭细胞数与低氧12、24、36h组比较差异显著(P<0.05),且低氧引起的细胞侵袭力的提高呈时间依赖性,随着低氧时间延长,侵袭细胞数逐渐增多,组间比较有显著差异(P<0.05);低氧时HPA蛋白表达的变化与细胞侵袭力变化呈正相关(r=0.8530,P<0.05)。结论:低氧可提高SKOV3细胞HPA的表达及细胞的侵袭力,且侵袭力的增加可能与HPA表达水平增高有关。AIM： To evaluate the effects of hypoxia on the expression of heparanase and the invasiveness of SKOV3 ovarial carcinoma cell line. METHODS： SKOV3 cells were incubated at either normoxia （37 ℃, 5% CO2, 21% O2） or hypoxia （37 ℃, 5%CO2, 1%O2） condition for 12 h, 24 h and 36 h. RT-PCR and Western blotting were used to detect mRNA and the protein expressions of heparanase under different conditions. Cell invasiveness was measured by matrigel invasion assay. RESULTS： Compared to normoxia group, the heparanase mRNA expression level in hypoxia group was increased and in 12 h hypoxia group was the highest. The heparanase protein expression in hypoxia group was also significantly increased （P 〈0.01 ） and the expression of heparanase in hypoxia group was also different （P 〈 0. 05）. Compared to normoxia group, the level of cell invasion was markedly increased in 12 h, 24 h and 36 h groups （P 〈0. 05）. During 12 - 36 h hypoxia period, the increase in hypoxia - induced invasiveness in SKOV3 cell line showed a time - dependent manner （P 〈 0. 05）. Meanwhile, there was a positive correlation between the expression of HPA and the invasiveness of SKOV3 cells （ r = 0. 8530, P 〈 0. 05 ）. CONCLUSION ： Invasion of SKOV3 cells in hypoxia condition correlates with heparanase level. Hypoxia plays an important role in the augmentation of the heparanase expression＇and the invasivehess of human ovarian cancer.

关 键 词：肝素酶 肿瘤侵润 低氧 卵巢肿瘤 

分 类 号：R711[医药卫生—妇产科学]