耐亚胺培南铜绿假单胞菌耐药相关基因的研究  

Investigation on the antimicrobial resistance related genes in imipenem-resistant Pseudomonas aeruginosa

作　　者：颜英俊[1] 刘华[1] 杨明清[1] 黄文方[1] 

机构地区：[1]四川省医学科学院四川省人民医院,成都610072

出　　处：《中国抗生素杂志》2008年第11期663-667,共5页Chinese Journal of Antibiotics

摘　　要：目的分析我院2005年1～12月临床分离34株耐亚胺培南铜绿假单胞菌对常用抗生素耐药相关基因存在状况，为临床抗感染治疗提供依据。方法应用PCR方法检测13种β-内酰胺类抗菌药物耐药相关基因，分别为blaTEM、blaSHV、blacCARB、blaoXA-10、blaPER、blaVEB、blaGES、blaDHA、blaIMP、blaVIM、blaGIM、blaSFM、oprD2；3种氨基糖苷类修饰酶基因，分别为：aac（6′）-I、aac（6′）-H和ant（2″）-I；整合酶基因I（intll）。结果耐药相关基因blaTEM、blaCARu、blaDHA、blaIMP、oprD2、aac（6′）-I、aac（6′）～II和ant（2″）-I、intI1阳性率分别为：5．9％、32．4％、14．7％、17．6％、97．1％、21．2%、33．3％、48．5％、3．0％；其余耐药基因均未检测到。结论携带多种耐药相关基因是耐亚胺培南的铜绿假单胞菌对多种抗生索耐药的重要原因之一，膜孔蛋白基因oprD2缺失不是本组铜绿假单胞菌对亚胺培南耐药的主要原因。Objective To investigate 34 strains of the antimicrobial resistance related genes of imipenem-resistant Pseudornonas aeruginosa from patients from Jan to Dec 2005. Method Antimicrobial-resistance related genes were detected by PCR method, including blaTrM, blaSHV, blaCARB, blaoXA-10, blaPER, blaVEB, blaGES, blaDHA, blaIMP, blaVIM, blaGIM, blaSPM, oprD2, acc（6′）-I, aac（6′）-II, ant（2″）-I and intI1. Results The positive rate of antibiotics resistant related genes, named blaTEM, blaCARB, blaDHA, blaIMP, oprD2, acc （6′）-I, aac（6′）-II, ant（2″）-I and intI1 were 5.9%, 32.4%, 14.7%, 17.6%, 97. 1%, 21.2%, 33.3%, 48.5% and 3.0%, respectively. While blaSHV, blaOXA-10, blaPER, blaVEB, blaGES, blaVIM, blaGIM and blaSIM genes were not detected. Conclusion The tested strains which carried multiple resistant genes related to imipenem were one of the main cause of the Pseudomonas aeruginosa resistant to many antibotics, while void of oprD2 gene was not necessaryly the main cause of bacteria resistant to imipenem.

关 键 词：铜绿假单胞菌 亚胺培南 Β-内酰胺酶类 氨基糖苷类修饰酶 整合酶I 耐药基因 

分 类 号：R378.991[医药卫生—病原生物学]