长春新碱对大鼠小肠肌电及动力的影响  

Effect of vincristine on the myoelectric activity and motility of the small intestine in rats

作　　者：王磊[1] 周慧[1] 赵涛[1] 张薇[1] 李兆申[1] 高峻[1] 

机构地区：[1]上海长海医院野战内科实验室,200433

出　　处：《中华消化杂志》2008年第9期604-607,共4页Chinese Journal of Digestion

基　　金：“十一·五”全军医药卫生科研基金课题资助项目（06G059）

摘　　要：目的探讨抗肿瘤药长春新碱（VCR）对清醒大鼠小肠肌电和动力及相关机制的影响。方法SD大鼠72只，分为6组，A组为对照组（n=18），尾静脉注射0．9％氯化钠溶液；B组为VC敦尾静脉注射组，按给药剂量分为0．25mg／kg（n=6）、0．5mg／kg（n=6）和0．75mg／kg（n=18）组；C组为假手术组（n=6），仅剖腹，尾静脉注射0．9％氯化钠溶液；D组为假手术＋VCR 0．75mg／kg（n=6）；E组为双侧膈下迷走神经切断＋0．9％氯化钠溶液（n=6）；F组为双侧膈下迷走神经切断＋VCR0．75mg／kg（n=6）。观察用药后大鼠小肠的肌电及动力变化。采用生物机能实验系统记录小肠慢波频率、曲线下面积，移行性复合运动（MMC）周期、MMCHI相持续时间；测定小肠推进率，免疫荧光染色观察肠肌间神经元及Caial间质细胞的表达。结果VCR0．25mg／kg组对小肠动力无明显改变。0．5和0．75mg／kg组均出现小肠肌电活动异常，且随作用时期不同表现不同，静脉注射（51．00±14．27）min后MMC模式被连续性峰电取代，（78．33±13．08）min后MMC逐渐恢复，0．5和0．75mg／kg组MMC周期分别为（343．17±142．93）s和（302．67±66．67）s，较A组[（740．22±98．92）s]缩短（F=31．325，P〈0．01）。用药后第3天，0．5和0．75mg／kg组曲线下面积为（2．56±0．30）mV·s和（2．57±0．56）mV·s，较A组降低[（4．04±0．64）mV·s，F=11．442，P〈0．01]。用药第3天，VCR0．75mg／kg组小肠推进率为（33．59±1．43）％，较A组减慢[（60．34±2．41）％，t=23．360，P〈0．01]。迷走神经切断组用药当日仍保持MMC相模式，第3天出现不规则峰电活动及慢波节律紊乱，VCR组曲线下面积为（2．49±0．33）mV·s，较A组降低[（4．10±0．80）mV·s，t=4．549，P=0．001]。用药第3天，VCR0．75mg／kg组Caial间质细胞阳性面积比为（5．84±3．11）％，较A组减少[（24．90±1�Objective To investigate the effects of vincristine on myoelectric activity and motility of the small intestine in conscious rats and its mechanism. Methods Seventy-two SD rats were divided into six groups . The rats in control group were received 0.9%0 NaCl solution （n=18） . The rats in group B were injected with vincristine and subdivided into 0.25 mg/kg（n = 6）, 0.5 rag/kg（n = 6 ）and 0.75 mg/kg groups. The group C and D was false operation （n=6）and false operation plus injection with 0.75 mg/kg of vincristine（n= 6）, respectively. The group E and F was subdiaphragmatic vagotomy plus 0.9 % NaCl （n= 6 ）or subdiaphragmatic vagotomy plus 0. 75 mg/kg of vincristine （n=6）, respectively. The myoelectric activity and motility of the small intestine were recorded. The frequency and area under the curve of slow wave, the periodicity of the migrating myoelectric complex （MMC） and the duration of MMC Ⅲ were analyzed. The expressions of the myenteric neurons and interstitial cells of Cajal were evaluated by immunofluorescence stain. Results The myoelectric activity in 0.25 mg/kg group was not different from the controls, but it changed in 0. 5 mg/kg and 0. 75 mg/kg groups which correlated with the time of vincristine injection. The irregular spike activity arose and accompanied with disruption of the MMC at （51 ± 14.27） minutes , but recovered at （78. 33± 13. 08） minutes. The periodicity was shorter in 0. 5 mg/kg [（343.17±142.93）s ]and 0.75 mg/kg groups [（302.67±66.67）s] compared with controls [（740.22± 98.92）s, F= 31. 325, P〈0. 01]. Three days after vincristine administration, the area under the curve of slow wave decreased to （2. 56 ± 0. 30） mV · s in 0. 5 mg/kg group and （2. 57±0.56） mV· s in 0.75 mg/kg group compared with the controls（（4. 10±0. 80）mV · s ,F=11. 442,P〈0. 01）. The intestinal propulsive rate was lower in 0.75 mg/kg group compared with the controls [（33. 59±1. 43） vs（60. 34±2. 41） ],t=23. 36,P〈0.01]. The expressi

关 键 词：长春新碱 移行性复合运动 睡眠 慢波 迷走神经 

分 类 号：R686[医药卫生—骨科学]