雷贝拉唑抑制大鼠胃壁细胞泌酸功能的机制研究  

The inhibitory mechanism of rabeprazole on acid secretion of parietal cells in rats

作　　者：王莉[1] 邹晓平[1] 曹俊[1] 田觅[1] 

机构地区：[1]南京大学医学院附属鼓楼医院消化科,210008

出　　处：《中华消化杂志》2008年第9期617-620,共4页Chinese Journal of Digestion

摘　　要：目的探讨质子泵抑制剂雷贝拉唑对大鼠胃壁细胞泌酸功能的抑制作用。方法将72只SD大鼠分成对照组（0．9％氯化钠溶液）、雷贝拉唑低剂量组（10mg／kg）和雷贝拉唑高剂量组（20mg／kg），每组24只。分别在1、2、4、6、12和24h每组各处理4只大鼠。用氢氧化钠滴定法测定大鼠胃内pH值，比色法测定胃壁细胞内H^＋-K^＋-ATP酶活性，电镜下观察胃壁细胞超微结构的改变。结果与对照组（1．97±0．30）相比，雷贝拉唑低剂量组（3．37±0．97）和高剂量组（5．96±0．26）在给药后1h内胃液pH值显著升高（P〈0．01），壁细胞H＋^-K^＋-ATP酶活性明显抑制（3．28±0．41比1．47±0．27和0．92±0．07，P〈0．05），而且在给药12h的差异仍有统计学意义（P〈0．01）。电镜下胃壁细胞超微形态改变与胃液pH值和壁细胞H^＋-K^＋-ATP酶活性变化相符。雷贝拉唑低剂量组与高剂量组在抑酸作用和起效时间方面差异有统计学意义（P〈0．01）。结论壁细胞超微结构的变化和H^＋-K^＋-ATP酶活性能准确反映壁细胞的泌酸情况，雷贝拉唑能迅速强效抑制大鼠壁细胞的泌酸功能，且与剂量有关。Objective To investigate the inhibitory mechanism of rabeprazole on acid secretion of parietal cells in rats. Methods Seventy two Spraque-Dawley rats were divided into control group（normal saline）, low dose of rabeprazole group （rabeprazole 10 mg/kg） and high dose of rabeprazole group （rabeprazole 20 mg/kg） with 24 each. Four rats in each group were treated at 1 h, 2 h, 4 h, 6 h, 12 h and 24 h, respectively, pH value and activity of H^＋- K^＋- ATPase were tested by NaOH titrimetric method and chromatometry, respectively. The changes of ultrastrueture of parietal cells were observed by electron microscope. Results Compared with control group（1. 97 ± 0. 30）, pH value in low （3. 37 ± 0. 97）and high （5.96±0.26）dose groups were significantly inereased（P〈0.01）, and the activity of H^＋- K^＋-ATPase was inhibited obviously at 1 h after adrainistration（3.28±0.41 vs 1.47±0.27 and 0. 92± 0.07, P〈0.05）. Those differences could he found even at 12 h（P〈0.01）. The ultrastructural changes of parietal cells were consistent with the increase of pH value and decreased activity of H^＋- K^＋-ATpase. The significant difference was found in acid depression and excellent time between low and high dose of rabeprazole groups. Conclusions The ultrastructural changes of parietal cells and the activity of H^＋- K^＋- ATPase can accurately reflect the acid secretion in rats. Rabeprazole has powerful and rapid effects in inhibiting acid secretion which correlates with the dosage .

关 键 词：胃酸 壁细胞 胃 雷贝拉唑 

分 类 号：R686[医药卫生—骨科学]