肌红蛋白在灿烂甲酚蓝修饰电极上的可逆电子传递反应  

The Reversible Electron Transfer Reaction of Myoglobin at Brilliant Cresyl Blue Modified Platinum Gauze Electrode

作　　者：董绍俊[1] 朱毅暋 

机构地区：[1]中国科学院长春应用化学研究所电分析化学开放研究实验室

出　　处：《化学学报》1990年第6期566-570,共5页Acta Chimica Sinica

摘　　要：利用循环电位吸收法和电位阶跃计时吸收法在薄层电解池中研究了肌红蛋白在灿烂甲酚蓝(BCB)修饰电极上和BCB溶液中的电化学行为。实验表明肌红蛋白可以发生可逆的还原和氧化反应,完全还原和氧化分别需要20和100s,氧化还原反应的标准速率常数被估算为5.6×10~4cm·s^(-1),并且稳定性很好,没有蛋白质变性反应发生。用光谱电化学方法测得该反应的标准电极电位和电子转移数与肌红蛋白相符。光电子能谱实验表明肌红蛋白没有吸附在BCB修饰电极上。对BCB修饰电极促进肌红蛋白的电子转移机理作了初步探讨。The electrochemical behavior of myoglobin at a Brilliant Cresyl Blue (BCB) modified platinum gauze electrode and spiral pt wire in the BCB solution in optically transparent thin layer cell base been investigated by using cyclic potential-absorbance method and double potential step chronoabsorptometry. The results reveal a reversible electron transfer resection of myoglobin. Exhaustive reductive and oxidative electrolyses are achieved at the modified platinum surface in 20 and 100s respectively. The formal heterogenous electron transfer rate constant is estimated (K_s=1.87×10^(-2)cm·s^(-1). The stability of electrode reaction is improved, and no denaturation occurred. The formal electrode potential and electron transfer number of this reaction are determined by using spectroelectrochemical method, and the results are in agreement with literature. Myoglobin does not absorb at BCB modified platinum gauze electrode, which has been confirmed by ESCA. The mechanism for the heterogeneous electron transfer of myoglobin at BCB modified electrode is discussed.

关 键 词：肌红蛋白 可逆电子传递 

分 类 号：O629.73[理学—有机化学]