脐血间充质干细胞对T淋巴细胞增殖和激活影响的实验研究  

Study on the effect of umbilical cord blood mesenchymal stem cells on proliferation and activation of allogeneic T lymphocytes

作　　者：陶艳玲[1] 张颢[2] 

机构地区：[1]广西壮族自治区人民医院儿科,广西南宁530021 [2]广西壮族自治区人民医院血液科,广西南宁530021

出　　处：《中国实用儿科杂志》2008年第11期833-835,共3页Chinese Journal of Practical Pediatrics

摘　　要：目的研究脐血源间充质干细胞(UCB-MSCs)对T淋巴细胞激活和增殖的影响。方法2006—2007年广西壮族自治区人民医院从脐血中分离和培养间充质干细胞,流式细胞术检测其表面标志以鉴定纯度;用B r-du法测定细胞增殖率,观察UCB-MSCs对异体T淋巴细胞增殖的影响;流式细胞仪检测共培养体系中CD6+9细胞水平。结果从脐血获得的MSCs免疫表型分析显示,其不表达HLA-II抗原。对PHA刺激的脐带血T淋巴细胞,UCB-MSCs对其的增殖反应具有抑制作用,呈细胞数量剂量依赖性;UCB-MSCs与脐带血单个核细胞共培养体系中,CD+69细胞比例降低,与对照组比较差异有统计学意义(P<0.01)。结论UCB-MSCs抑制PHA刺激的T淋巴的增殖及激活,这种抑制特性表明,UCB-MSCs对免疫反应具负调节作用,可能为GVHD的防治提供一条新途径。Objective To study the effect of umbilical cord blood mesenchymal stem cells on T lymphocytes. Methods UC-MSC was separated from umbilical cord. The purity of MSC was analyzed by flow cytometry;the proliferation of allogeneie T lymphocytes was measured by Brdu method; the CD69 expression was detected by flow cytometry. Results The immunophenotypie analysis showed that UCB-MSCs expressed no HLA-Ⅱ ;UCB-MSCs could suppress umbilical cord blood lymphocytes proliferation stimulated by mitogen in dose-dependent manner; CD69^＋ suppopulation was decreased （ P 〈 0.01 ）. Conclusion UCB-MSCs can supress T cells proliferation and activation,which indicates UCB-MSC might have negative immunomodulation and be useful in the prevention of GVHD in allogeneic stem ceils.

关 键 词：间充质干细胞 脐血 Brdu法 T淋巴细胞 

分 类 号：R72[医药卫生—儿科]