曙红B-盐酸氯米帕明体系的光谱特性及其分析应用  

Study on spectral characteristics of eosin B-clomipramine hydrochloride system and its analytical application

作　　者：郭立珉[1] 冯素玲[1] 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007

出　　处：《分析试验室》2008年第11期10-13,共4页Chinese Journal of Analysis Laboratory

基　　金：河南省新乡市科技发展(07G052)项目资助

摘　　要：在弱酸性介质中,盐酸氯米帕明能和曙红B通过静电作用、疏水作用形成离子缔合物,从而引起曙红B溶液的吸光度、荧光强度下降,共振光散射显著增强;其变化值均与盐酸氯米帕明在一定浓度范围呈良好的线性关系。建立了共振光散射法测定盐酸氯米帕明的新方法,线性范围为0.025-2.5 mg/L,已用于药物中盐酸氯米帕明的测定,回收率在100.2%-104.4%。In a weak acid medium, clomipramine hydrochloride can react with eosin B to form an ion-association complex by virtue of electrostatic and hydrophobic interaction. As a result, the absorbance and the intensity of fluorescence decreased, at the same time, the resonance light scattering enhanced significantly, which were linear with the concentration of clomipramine hydrochloride, respectively. A novel method for the determination of clomipramine hydrochloride was developed, with a linear range of 0. 025 - 2.5 mg/L. The method was successfully applied to the determination of clomipramine hydrochloride in pharmaceutical. The recoveries were in the range of 100.2% - 104.4%.

关 键 词：共振光散射 盐酸氯米帕明 曙红B 吸收光谱 荧光光谱 

分 类 号：O657.3[理学—分析化学]