分子荧光法测定水中的微量铁  

Determination of micro-iron by molecular fluorescence in water

作　　者：王修中[1] 李素芳[1] 于宁[2] 姚文红[1] 

机构地区：[1]青岛农业大学理学院,青岛266109 [2]青岛农业大学招生就业处,青岛266109

出　　处：《分析试验室》2008年第11期88-90,共3页Chinese Journal of Analysis Laboratory

摘　　要：基于Fe（Ⅱ）与邻菲口罗啉生成络合物使其荧光猝灭的特性,讨论了在室温下,pH 5.5的乙酸-乙酸铵的缓冲介质中,以λex=270 nm为激发波长,于eλm=365 nm波长处测定该络合物荧光强度。结果表明Fe（Ⅱ）质量浓度在0.8-120.0 ng/mL范围内与络合物的荧光强度具有良好的线性关系,相关系数0.9994,检出限0.50 ng/mL。对60 ng/mL的Fe RSD（n=9）为2.8%。Fe的回收率为98.03%-102.9%。方法已用于水中微量铁的测定。Based on the formation of Fe（ Ⅱ ）-phen complex which can reduce the fluorescence signal of phenanthroline, the determination of micro-iron in environmental water by fluorimetry is discussed in this paper. The experiments were carried out in the pH 5.5 acetic acid-acetate ammonium buffer solution at room temperature; and the complex excitation and emission wavelengths are 270 nm and 365 run, respectively. Under optimum conditions, the fluorescence signal is linear with iron concentration in a range from 0.80 ng/mL to 120 ng/mL （R = 0. 9994）. The detection limit of the method is 0.50 ng/mL for iron. The relative standard deviation of the method is 2.8% （ n = 9） for 60 ng/mL iron. The recoveries of iron by the method are 98.03% - 102.9%. The method has been applied to the determination of micro-iron in water with satisfactory results.

关 键 词：FE 邻菲口罗啉 荧光猝灭 水 

分 类 号：O657.3[理学—分析化学]