海洋微藻抗肿瘤及抗氧化活性初筛  

Primary screening of marine microalgae with antitumor and antioxidative activities in vitro

作　　者：李婷[1] 刘培培[1] 卢圳域[1] 郭健[2] 顾谦群[1] 朱伟明[1] 

机构地区：[1]海洋药物教育部重点实验室中国海洋大学医药学院,山东青岛266003 [2]中国海洋大学水产学院,山东青岛266003

出　　处：《中国海洋药物》2008年第5期18-22,共5页Chinese Journal of Marine Drugs

基　　金：中国海洋大学人才基金资助项目

摘　　要：目的对90株海洋微藻的提取物进行了体外抗肿瘤和抗氧化活性筛选,从中寻找活性微藻藻株。方法以细胞凋亡诱导、细胞坏死以及自由基清除为活性指标,采用SRB,TLC自显影技术和DPPH方法进行抗肿瘤以及抗氧化活性筛选,并对活性藻株代谢产物进行了HPLC-UV分析。结果与结论发现5株微藻的提取物具有抗肿瘤活性、1株微藻的提取物具有抗氧化活性。HPLC-UV分析表明,其抗氧化活性可能与具有400nm最大紫外吸收的化合物有关。Objective In order to search for active marine mieroalgae, the antitumor and anti- oxidative activities of 90 strains of marine microalgae were screened. Methods Antitumor and antioxidation were assayed using SRB, TLC bioautography and DPPH methods, respectively. Results and Conclusion The extracts of the mycelium of 5 strains of microalgae showed cytotoxicity against P388 cells. And the extract of one strain of microatgae exhibited antioxida tion against DPPH radical and UV-radiation, which showed a series of UV absorptions at 400nm in the HPLC-UV analysis.

关 键 词：微藻 抗肿瘤 细胞凋亡 抗氧化 1 1-二苯基苦基肼自由基 薄层色谱自显影 

分 类 号：Q949.2[生物学—植物学] R965[医药卫生—药理学]