栉孔扇贝裙边糖胺聚糖对增殖的血管平滑肌细胞c-myc和TNF-α mRNA水平的影响  

Effects of scallop skirt glycosaminoglycan on the expression of c-myc and TNF-α genes in proliferative vascular smooth muscle cells

作　　者：沙丽颖[1] 杨英[1] 殷毅[2] 丁守怡[3] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003 [2]北京师范大学生命科学院,北京100875 [3]青岛大学医学院微生物教研室,山东青岛266021

出　　处：《中国海洋药物》2008年第5期41-45,共5页Chinese Journal of Marine Drugs

摘　　要：目的观察栉孔扇贝(Chlamys farreri)裙边糖胺聚糖(SS-GAG)对体外培养的血管平滑肌细胞(VSMC)内原癌基因c-myc mRNA、肿瘤坏死因子(TNF-α)mRNA水平的影响,以探讨SS-GAG抗动脉粥样硬化(AS)的作用机理。方法建立碱性成纤维细胞生长因子(bFGF)诱导的平滑肌细胞增殖模型,采用非同位素原位杂交方法测定SS-GAG对增殖的VSMC内c-myc mRNA、TNFαmRNA水平的影响。结果低、高剂量SS-GAG组c-myc、TNF-αmRNA水平明显低于模型组(P<0.01)。结论SS-GAG对bFGF诱导增殖的VSMC内c-myc、TNF-α基因的表达均有抑制作用,且随着SS-GAG浓度的升高抑制作用增强。Objective To observe the effects of Chlamys farreri skirt glycosaminoglycan（SS- GAG） on the expressions of c-myc and TNF-α genes so as to explore the anti-atherosclerosis （AS） mechanism of SS-GAG. Methods The cell proliferation model of vascular smooth muscle cells （VSMC） was established by basic fibroblast growth factor （bFGF） induetion. In Situ hybridization marked by non-isotope was applied to determine the effects of SS-GAG on the mRNA levels of c-myc and TNF in proliferative VSMC. Results The mRNA levels of c- myc and TNF in low dose group and high group of SS-GAG were obviously lower than that of model group （P〈0.01）. Conclusion SS-GAG has inhibitory effect on both c-myc and TNF gene expression in proliferative VSMC induced by bFGF and the effect is concentration dependent.

关 键 词：栉孔扇贝 糖胺聚糖 肿瘤坏死因子 动脉粥样硬化 原位杂交 

分 类 号：Q915.817[天文地球—古生物学与地层学] R965[生物学—古生物学]