过敏性休克中干扰素-γ表达的意义研究  

Significant study on the protein expression of IFN-γ in anaphylactic shock

作　　者：李振宇[1] 梁艳冰[1] 唐皓[1] 罗斌[2] 陈志斌[1] 林贤寿[1] 马中富[1] 

机构地区：[1]中山大学附属第一医院普内科,广州510080 [2]中山大学基础医学院法医教研室

出　　处：《中国临床实用医学》2008年第11期3-5,共3页China Clinical Practical Medicine

基　　金：基金资助：广东省科技计划项目（项目编号：2004B30601002）;天津红日药业股份有限公司资助（项目编号：200708）;创伤、烧伤与复合伤国家重点实验室开放基金（项且编号：200711）

摘　　要：目的观察过敏性休克致死患者心脏、肺脏、肾脏、肝脏和脾脏中干扰素-γ蛋白水平的表达变化，探讨在过敏性休克中可能的作用机制。方法收集2006年2月至2007年10月经法医鉴定中心鉴定，尸体解剖及组织学检验符合过敏性休克患者12例（男7例，女5例，年龄3-65岁）。取其心脏、肺脏、肾脏、肝脏和脾脏组织；随机收集同期15例无病外伤所致的意外死亡者（男8例，女7例，年龄12-55岁）同类脏器组织作为正常对照。HE染色行病理检查，采用免疫组化法检测IFN-γ表达部位。结果病理切片HE示：过敏性休克组中可见各脏器的广泛淤血，间质血管充血，肺脏可见嗜酸性粒细胞。肾脏肾小球部分玻璃样变性或纤维化。肾小管上皮轻度自溶。肝脏部分肝细胞脂肪变性。各脏器中IFN-γ表达水平均较正常组增高，差异具有统计学意义。结论IFN-γ可能参与过敏性休克的病理生理过程。Objective To investigate the changes of protein expression levels of IFN-γ in the heart, lungs, kidney, liver and spleen from patients who died of allergic shock to discuss the possible mechanism of allergic shock in vivo. Methods Twelve cases who died of allergic shock confirmed by autopsy and histologic examination performed by verification centre of forensic medicine of Sun Yat-sen university from Feb, 2006 to Oct, 2007 were enrolled, including 7 males and 5 females aging from 3-65 years. Tissues from heart, lung, kidney, liver and spleen were taken. Samples from the same organs of fifteen healthy cases died of accidence were taken as normal control, including 8 males and 7 females aging from 12-55 years. Histochemistry was used to determine the protein expression site of IFN-γ in the tissues. Image analysis system and statistical methods were used to compare the photodensity and positive cell numbers. Statistical analysis was performed using SPSS software package. Results In the allergic shock group, tissue sections showed widely distributed congestion in all organs, interstitial vessel congestion, eosinophile granulocytes in lungs, partial hyalinization or fibrosis of renal glomeruli, slight autolysis of epithelia of renal tubules and fatty degeneration of a portion of hepatic cells. The protein levels of IFN-γ in all organs of the cases in the allergic shock group were both higher than those in the normal control group （ P 〈 0.05 ）, The differences were of statistical significance. Conclusion The present study suggesting that IFN-γ may be involved in the regulation of development of allergic shock.

关 键 词：过敏性休克 干扰素Γ 病理生理 

分 类 号：R593.1[医药卫生—内科学] R512.62[医药卫生—临床医学]