溶原性噬菌体对铜绿假单胞菌生物被膜形成的影响  

The Influence of Lysogenic Phage on Biofilm Formation of Pseudomonas aeruginosa

作　　者：杜宝中[1] 曾蔚[2] 陶传敏[3] 康梅[3] 刘梅[2] 张再蓉[2] 贾文祥[2] 

机构地区：[1]西藏大学医学院病原生物学教研室,拉萨850000 [2]四川大学华西基础医学与法医学院微生物学教研室 [3]四川大学华西医院实验医学科

出　　处：《四川大学学报（医学版）》2008年第6期886-889,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30370079);纽约中华医学基金会(CMB82-412)资助

摘　　要：目的研究铜绿假单胞菌(PA3094)溶原性噬菌体Ppa3094对该菌生物被膜形成的影响。方法采用平板培养法建立PA3094及PA3094-L(带前噬菌体Ppa3094的溶原性菌株)两株菌的体外生物被膜模型;MTT法测定生物被膜形成过程中两株菌的生物被膜活菌量;实时荧光定量PCR测定两株菌在培养不同时间点藻酸盐合成基因algC、algD的表达量。结果两株菌均在培养5～7 d时形成成熟的生物被膜,呈薄膜状。在生物被膜形成过程中,两株菌的生物被膜菌量存在明显差异,在整个生物被膜形成过程中,PA3094生物被膜菌量均比PA3094-L高;PA3094-L在培养第5 d时生物被膜菌量达最高,第7 d有所下降。随着培养时间的延长,藻酸盐合成基因algD、algC表达量均上调,但PA3094-L表达量均比PA3094低,且algC表达量差异更大。结论铜绿假单胞菌溶原性噬菌体Ppa3094可降低藻酸盐合成基因algD、algC的表达,从而影响生物被膜的形成。Objective To investigate the effect of the lysogenie phage Ppa3094 on the blofilm formation of PA3094. Methods The modified plate culture method was used to established the biofilm model in vitro. The viable counts of bacteria in biofilm were detected by MTT method ;The real-time RT-PCR was applied to measure the expression level of algC and algD during the biofilm formtion of PA3094 and PA3094-L. Results Biofilm of both strains were mature at 5th to 7th day. The structures of the hiofilms were both like pellicle. There was a significant difference in the viable counts of bacteria during biofilm development between PA3094 and PA3094-L. The expression of algC and algD genes was upregulated during biofilm formation. However, the expression level of PA3094-L was lower than PA3094, especially algC at 12 h. Conclusion The lysogenie phage Ppa3094 could influence the biofilm formation during its development through changing the expressing level of the alginate biosynthetic genes.

关 键 词：溶原性噬菌体 铜绿假单胞菌 生物被膜 藻酸盐 基因表达 

分 类 号：R378[医药卫生—病原生物学]