4株野鸭源H5N1亚型禽流感病毒的全基因组鉴定  

Characterization of the complete genome of four avian influenza viruses H5N1 isolated from mallard

作　　者：唐应华[1,2] 吴培培[1] 彭大新[1] 张评浒[1] 龙进学[1] 张文俊[1] 李彦芳[1] 刘秀梵[1] 

机构地区：[1]扬州大学兽医学院,农业部畜禽传染病学重点开放实验室,扬州225009 [2]江苏省农科院国家兽用生物制品工程技术研究中心,南京210014

出　　处：《中国人兽共患病学报》2008年第11期1006-1008,1013,共4页Chinese Journal of Zoonoses

基　　金：国家863项目:(2006BAA10A205);江苏省高技术研究:(BG2007336)

摘　　要：目的为了解H5N1病毒对基因进化情况,方法对4株野鸭源的H5N1禽流感病毒(A/mallard/Huadong/S/2005(S),A/mallard/Huadong/lk/2005(lk),A/mallard/Huadong/Y/2003(Y),A/mallard/Huadong/hn/2005(hn))进行全基因组测序分析,并鉴定对非免疫麻鸭的毒力。结果4株病毒全基因组序列及其氨基酸序列无明显差异,仅在HA裂解位点区,S和lk病毒的322位是Leu,329位缺失,而Y和hn在此两处分别是Gln和Lys。在遗传距离上,Y和hn比较接近,S与lk比较接近。结论根据H5亚型病毒最新分类规则,S和lk的与clade 2.3.4一致,而Y属于clade 2,hn位于clade 3。对麻鸭致病性试验表明,Y和hn是低致病性病毒,而S及lk是高致病性病毒。HA的322位的Leu和329位缺失可能是clade 2.3.4这类病毒的一个遗传进化标志。To investigate the genetic variation on phenotype of HSN1 avian influenza virus（AIV）, the complete genomes of four H5N1 AIVs（A/mallard/Huadong/S/2005 （S）, A/mallard/Huadong/lk/2005 （lk）, A/mallard/Huadong/Y/2003 （Y）, A/mallard/Huadong/hn/2005 （hn）） were sequenced and the pathogenicity of these viruses were characterized in non-immunized mallards （Anas platyrhynchos）. Results showed that the key amino acid residues were conserved among these viruses except the difference in HA cleavage region, S and lk viruses with Leu322 and deletion at 329 （-329）, whereas Y and hn viruses with Gln322 and Lys329. The homology of these viruses showed that S closely related to lk, while Y related to hn. In term of the recently established unified nomenclature system of H5N1 virus clade based upon the evolution of the HA gene, S and lk viruses grouped into clade 2.3.4, Y virus located at elade 2,and hn attributed to elade 3. The Y and hn viruses were non-pathogenic but S and lk were highly pathogenic in mallards. All these study data indicated that Virus phenotype variation in mal- lards exhibited difference genetic evolution or, conversely, virus phenotype similarity in mallards had closely related to genetic distance. The Leu322 and -329 in HA gene could be served as genetic evolution mark for virus of clade 2.3.4.

关 键 词：禽流感病毒 野鸭 遗传进化 

分 类 号：S852.65[农业科学—基础兽医学]