微RNA-21在乳腺浸润性导管癌中的表达及与磷酸酶张力蛋白基因的相关分析  

Expression of microRNA-21 in invasive ductal carcinoma of the breast and its association with phosphatase and tensin homolog deleted from chromosome expression and clinicopathologic features

作　　者：黄关立[1] 张筱骅[1] 郭贵龙[1] 黄卡特[2] 杨开颜[2] 胡孝渠[1] 

机构地区：[1]温州医学院附属第一医院肿瘤外科,温州325000 [2]温州医学院附属第一医院病理科,温州325000

出　　处：《中华医学杂志》2008年第40期2833-2837,共5页National Medical Journal of China

基　　金：基金项目：浙江省自然科学基金资助项目（Y207301）

摘　　要：目的探讨微RNA-21（mir-21）和第10染色体同源丢失性磷酸酶张力蛋白基因（PTEN）在乳腺浸润性导管癌中的表达情况及临床意义。方法采用茎环实时荧光逆转录聚合酶链反应（realtime RT-PCR）检测40例乳腺浸润性导管癌及其对应的癌旁正常乳腺组织中mir-21表达，免疫组化Envision法检测相应癌组织中PTEN表达，分析mir-21表达和PTEN表达的相关性及其与临床病理特征的关系。结果茎环realtime RT-PCR检测mir-21表达的敏感性和特异性良好；mir-21在乳腺癌组织中的表达显著高于正常组织（P〈0．001）；PTEN在癌组织中的低表达率为45％（18／22）。PTEN低表达组mir-21的表达显著高于PTEN高表达组（P=0．013）。mir-21的高表达还和较高的TNM分期、淋巴结转移及高增殖指数有关（P值分别为0．021，0．010和0．030）。结论mir-21在乳腺浸润性导管癌中的表达升高，可能和乳腺癌的增殖、侵袭转移有关；在乳腺癌中PTEN可能为其靶基因之一。Objective To investigate the expression of microRNA-21 （ mir-21 ） in invasive ductal carcinoma （IDC） of the breast and its association with phosphatase and tensin homologue deleted from chromosome （PTEN） -10 protein expression and the clinicopathologic features of IDC. Methods Specimens of IDC and normal tissues more than 5 cm away from the tumor tissues were collected from 40 IDC patients, all female, aged 53 （35 -77 ）. Stem-loop real-time RT-PCR was used to examine the mir-21 expression. Immunohistochemistry was used to examine the PTEN protein expression in the tumor tissue. The association of mir-21 expression with the PTEN expression and the clinicopathologic features of the breast IDC were analyzed. Results Compared with the nontumor control samples, the median （M） of relative expression of mir-21 （2^-△△Ct） was 5. 770 （25th-75th percentile, 3. 605 -7. 255 ） in the tumor samples, significantly higher than that of the nontumor control samples （ set at 1. 000, P 〈 0. 001 ）. Reduced PTEN protein expression was seen in 45% （18/22） of all cases. The expression of mir-21 was higher in the group of reduced PTEN expression （with an M of 6. 800） than in the group of high PTEN expression （with an M of 4.850, P =0.013）. The up-regulated expression of mir-21 was positively correlated with the TNM clinical stage, lymph node positivity, and proliferation index （ P = 0. 021, 0. 010, and 0. 030 respectively） ： Conclusion mir-21 plays an important role in the development and progression of breast cancer. PTEN is possibly one of the targets of mir-21.

关 键 词：乳腺肿瘤 微RNA 微RNA-21 PTEN磷酸水解酶 

分 类 号：R686[医药卫生—骨科学]