小檗碱对脂质代谢相关基因PPARα和CPTIA表达的影响  

Effect of Berberine on the expression of lipid metabolism-associated gene PPARα and CPTIA

作　　者：师凌云[1,2] 田蜜[1,2] 常伟[1,2] 袁野[1,2] 周岐新[1] 

机构地区：[1]重庆医科大学药理学教研室 [2]重庆市生物化学及分子药理学重点实验室,重庆400016

出　　处：《中国药理学通报》2008年第11期1461-1464,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30572353)

摘　　要：目的研究小檗碱(Berberine,Ber)对HepG2细胞的PPARα基因及其调控基因CPTⅠA表达的影响。方法以PPARα激动剂非诺贝特作阳性对照药,采用RT-PCR法研究Ber对HepG2细胞PPARα基因及其调节基因CPTⅠAmR-NA表达影响的时—效和量—效关系。结果非诺贝特明显促进CPTⅠAmRNA表达;Ber也呈浓度和时间依赖性促进CPTⅠAmRNA的表达,而对PPARαmRNA表达无影响;PPARα特异拮抗剂MK-886能阻断非诺贝特和Ber上述作用。结论Ber可能通过激动PPARα促进与脂质代谢相关的靶基因CPTⅠAmRNA的表达。Aim To investigate the effect of Ber on the expression of PPARα gene and its regulatory gene CPTI A in HepG2. Methods RT-PCR was taken to evaluate the concentration-effect and time-effect of Ber on the expression of PPARα gene and its regulatory gene CPT I A mRNA in the cultured HepG2 ceils. Results It was shown that a PPARα agonist, FF significantly promoted CPT I A mRNA expression;Ber also promoted cFr I A mRNA expression in a concentration- and time-dependent manner, but had no effect on the ex-pression of PPARα mRNA. Specific PPARα antagonist MK-886 could block the roles of FF and Ber mentioned above. Conclusion Ber might promote the expression of lipid metabolism-associated gene CPT I A mRNA through stimulating PPARα.

关 键 词：小檗碱 HEPG2 PPARΑ CPTⅠA 脂质代谢 

分 类 号：R284.1[医药卫生—中药学] R329.2[医药卫生—中医学]