HPLC-电化学检测法用于黄芩苷和黄芩素大鼠血浆稳定性研究  

Study on stability of baicalin and baicalein in mouse plasma by HPLC-ECD

作　　者：汪祺[1] 张玉杰[1] 李维峰[1] 杨洁[1] 姜昊[1] 孙万晶[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国中药杂志》2008年第22期2675-2678,共4页China Journal of Chinese Materia Medica

摘　　要：目的:考察黄芩苷(BG)和黄芩素(B)在缓冲液及大鼠血浆中的稳定性,建立测定二者在大鼠血浆中含量的样品处理方法,并初步探讨二者在血浆中稳定性的影响因素。方法:采用HPLC-电化学检测法,分别考察BG,B在不同条件下的稳定性及使二者稳定的条件。结果:BG,B在大鼠血浆中较在pH7.4的缓冲液中更不稳定。在pH7.4缓冲液中加入抗氧化剂可使BG和B的稳定性明显提高,剩余百分含量达到102%和100%;在大鼠血浆中加入抗氧化剂及1 mol.L-1HCl可使BG和B的稳定性明显增加,剩余百分含量可分别达到98%和103%。结论:BG和B在pH7.4缓冲液中的稳定性主要受氧化影响,BG和B在血浆中的稳定性不仅与氧化有关,还受血浆中的内源物质影响,最终确定每1 mL大鼠血浆中加入抗氧剂及100μL1 mol.L-1HCl为血浆样品的处理条件,此条件可使样品稳定。Objective： To compare the stability of baicalin and baicalein in buffered aqueous solutions and mouse plasma in order to discuss the degradation mechanism and the method of disposing samples. Method： HPLC-ECD was used to determine baicalin and baicalein in different conditions, and the disposing samples methods were selected to stabilize them. Result： Baicalin and baicalein in the buffered aqueous solution at pH 7.4 was more stable than that in the mouse plasma. After adding Vit C in the buffered aqueous solution, the stability of baicalin and baicalein could be improved obviously and their remained percents were 102% and 100%. The stability of baicalin and baicalein could be enhanced distinctly in the mouse plasma only when adding Vit C and 1 mol · L^-1 HCl, and furthermore their remained percentage was up to 98% and 103%. Conclusion： The stability of baicalin and baicalein is related to the oxidation and enzyme degradation and it can be improved by adding Vit C and 1 mol · L^-1 HCl in the solution.

关 键 词：HPLC-电化学 黄芩苷 黄芩素 血浆中稳定性 

分 类 号：R285.5[医药卫生—中药学]