骨髓间充质干细胞通过血管内皮细胞生长因子受体2调控神经干细胞的增殖与分化  

Mesenchymal stem cells regulate the proliferation and differentiation of neural stem cells through vascular endothelial growth factor receptor 2

作　　者：邓志锋[1] 汪泱[2] 刘诸敏[1] 涂伟[1] 赖贤良[1] 娄远蕾[2] 

机构地区：[1]南昌大学第二附属医院神经外科,330006 [2]南昌大学第一附属医院泌尿外科研究所

出　　处：《中华实验外科杂志》2008年第11期1460-1462,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30560156）;江西省自然科学基金资助项目（0540087）

摘　　要：目的观察血管内皮细胞生长因子受体2（VEGFR2）在骨髓间充质干细胞（MSCs）调控神经干细胞（NSCs）增殖与分化中的作用。方法细胞共培养分为5组：NSCs＋MSCs、NSCs＋MSCs＋SU5416（VEGFR2阻断剂）、NSCs＋HMVECs、NSCs＋HMVECs＋SU5416、NSCs＋3T3。共培养6h后，采用逆转录-聚合酶链反应检测各组NSCs标志物nestin、成熟星形胶质细胞标志物GFAP及Notch信号分子Notch1和hes1的表达。结果在MSCs及HMVECs共培养组中nestin的表达较高，当VEGFR2信号通路被阻断后，nestin的表达明显下降，而成熟神经细胞GFAP的表达却明显增加。同时，在MSCs及HMVECs共培养组中，Notch1、hes1的表达也明显高于313对照组及VEGFR2信号通路阻断剂组。结论VEGFR2在MSCs调控NSCs增殖与分化过程中可能起重要作用。Objective To study the effect of vascular endothelial growth factor receptor 2 （ VEG- FR2） on proliferation and differentiation of neural stem cells （NSCs） regulated by mesenchyma1 stem cells （MSCs）. Methods In co-culture experiments, NSCs were co-cultured with MSCs, human microvascular endothelial cells （HMVECs） or NIH3T3. To exclude the effect of VEGFR2,SU5416 （ a selective inhibitor of the tyrosine kinase activity of the VEGFR2） was applied in co-culture system. Six h later, the expression of nestin and GFAP was detected by RT-PCR. Results RT-PCR studies revealed that the expression of nestin in NSCs was up-regulated by MSCs and HMVECs, whereas the level was sharply down-regulated when VEGFR2 was blocked, and the expression of GFAP was increased. Determination of Notchl and Hesl in NSCs showed that MSCs and HMVECs promoted the expression of Notchl and Hesl. Conclusion VEGFR2 may play an important role in the proliferation and differentiation of NSCs regulated by MSCs.

关 键 词：骨髓 间充质干细胞 神经干细胞 增殖 分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]