氯化锂联合自体激活雪旺细胞治疗脊髓损伤  

The study on repair of spinal cord injury by cografting auto logous activated schwann cells and lithium chloride

作　　者：冯世庆[1] 纪江峰[1] 孔晓红[3] 曹富江[1] 陈家童[2] 

机构地区：[1]天津医科大学总医院骨科,300052 [2]天津南开大学生命科学院 [3]天津南开大学医学院

出　　处：《中华实验外科杂志》2008年第11期1496-1498,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30872603）;教育部新世纪人才支持计划资助项目（NCET-06-025150）;天津市应用基础与前沿技术计划资助项目（07JCYBJC102008）;天津市卫生局科研基金资助项目（06ky433）

摘　　要：目的观察氯化锂联合移植自体激活雪旺细胞（AASCs）在治疗脊髓损伤（SCI）中的作用。方法Wistar成鼠48只建立T10急性SCI模型。随机均分为4组：AASCs移植组、氯化锂移植组、氯化锂联合AASCs组和DMEM移植组。术后行体感诱发电位（SEP），运动诱发电位（MEP）检查和BBB评分。12周后进行10％BDA神经顺行示踪标记，切片后，行Cy3荧光探针染色、免疫组织化学及苏木素-伊红（HE）染色。结果术后4周BBB评分各组之间差异开始有统计学意义（F=46．897，P〈0．05）。实验结束时，SEP、MEP波幅值各组间差异有统计学意义（F-SEP=15．47，P〈0．05；F—MEP=8．974，P〈0．05）；BDA示踪，HE和免疫组织化学染色各组间差异有统计学意义（P〈0．05）。结论氯化锂联合AASCs移植可以显著改善SCI后的功能恢复。Objective To discuss the curative effectivenss of lithium chloride in combined with autologus activated Schwann cells （AASCs） in the treatment of spinal cord injury （SCI） in rats. Methods SCI models in 48 adult Wistar rats were made at T10 and divided into 4 groups： AASCs group,lithium chloride group, AASCs ＋ lithium chloride group and DMEM group. After surgical operation, SEP, MEP and BBB score were made in every week. 10% BDA anterograde tracing mark, Cy3 fluorescence staining, HE staining and immunohistochemistry staining were made 12 months later. Results On the 4th week after grafting, there was statistically significant difference in BBB score between groups （ANOVA, F = 46. 897, P 〈 0.05 ）. At the end of the experiment, there was statistically significant difference in in SEP and MEP amplitudes of wave between groups （ F-SEP = 15.47, P 〈 0.05 ; F-MEP = 8. 974, P 〈 0.05 ）. Statistically significant difference was found in BDA tracing, HE and immunohistochemistry staining （ P 〈 0.05 ）. Conclusion Evident amelioration after SCI can be found by co-transplanting AASCs and lithium chloride.

关 键 词：雪旺细胞 氯化锂 脊髓损伤 再生 

分 类 号：R651.2[医药卫生—外科学]