透明颤菌血红蛋白在产聚γ-谷氨酸地衣芽胞杆菌WX-02中的表达  

Expression of Vitreoscilla Hemoglobin in Poly γ-glutamic Acid-producing Bacillus licheniformis WX-02

作　　者：熊欢[1] 魏雪团[1] 冀志霞[1] 孙明[1] 陈守文[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《微生物学通报》2008年第11期1703-1707,共5页Microbiology China

基　　金：教育部新世纪人才支持计划(No.NCET-07-0341);国家"863计划"项目(No.2008AA10Z317)

摘　　要：聚γ-谷氨酸(γ-PGA)是一种应用前景良好的生物高分子材料。通过构建含有α-淀粉酶(amyE)基因两端交换臂的整合载体pDG1730-vgb,将透明颤菌血红蛋白基因(vgb)整合到聚γ-谷氨酸生产菌株地衣芽胞杆菌(Bacillus licheniformis)WX-02染色体中,获得重组子M2。一氧化碳差光光谱结果验证M2中表达了有活性的血红蛋白,3 L发酵罐分批发酵结果显示M2的生物量比出发菌株WX-02提高了25.5%,γ-PGA产量提高了20%。Poly γ-glutamate is a biopolymer material that has a good application prospect.The Vitreoscilla hemoglobin（VHb） gene was integrated into the chromosome of Bacillus licheniformis WX-02 by integrative vector pDG1730-vgb.The expression of VHb was confirmed by CO-difference spectra analysis.It was shown by the results of batch cultures in a 3 L bioreactor that biomass and γ-PGA obtained in the recombinant M2 were 25.5 % and 20% higher than those of the control respectively.

关 键 词：地衣芽胞杆菌 聚Γ-谷氨酸 透明颤菌血红蛋白 

分 类 号：Q78[生物学—分子生物学] TQ324.9[化学工程—合成树脂塑料工业]