烟草炭疽病拮抗生防菌的筛选  

Screening of Antagonistic Strains to Tobacco Anthracnose

作　　者：万秀清 郭兆奎 颜培强 李洪林 李丽杰 于红梅 

机构地区：[1]黑龙江省烟草科学研究所,牡丹江157011 [2]牡丹江市北山公园,牡丹江157011

出　　处：《微生物学通报》2008年第11期1727-1731,共5页Microbiology China

基　　金：黑龙江省烟草专卖局资助项目“烟草病虫害生物防治技术研究”(No.hykh20030001)

摘　　要：本文应用荧光假单胞杆菌的16S-23S ribosomal RNA序列以及部分抗菌素合成基因序列的PCR鉴定引物,对从烟草根际分离到的400份细菌进行了鉴定,结果获得了21份含Prn(硝吡咯菌素)合成酶基因的荧光假单胞杆菌和1份含有DAPG(2,4-二乙酰基藤黄酚)合成酶基因以及PCA(Phenazine-1-carboxylic acid,吩嗪-1-羧酸)的荧光假单胞杆菌。室内实验结果表明,含DAPG和PCA的荧光假单胞杆菌对烟草炭疽病具有明显拮抗作用。Special PCR primers were designed from the sequence of 16S-23S ribosomal RNA of Pseudomonas fluorescens,then these primers were used to screening 400 bacteria strains which selected from tobacco planting soil.In the experiment,21 Pseudomonas fluorescens strains which contain Pyrrolnitrin had been identified,and 1 Pseudomonas fluorescens strain which contain 2,4-diacetylphloroglucinal and Phenazine-1-carboxylic acid had been identified,too.The results showed that the strain which contain 2,4-diacetylphloroglucinal and Phenazine-1-carboxylic acid inhibit to tobacco anthracnose.

关 键 词：荧光假单胞杆菌 烟草炭疽病 生防 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]