一株家养野猪源猪圆环病毒2型的分离与鉴定  

Isolation and Identification of Porcine Circovirus Type 2 Virus from a Domesticated Wild Boar

作　　者：王海燕[1] 高崧[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,扬州225009

出　　处：《微生物学通报》2008年第11期1760-1763,共4页Microbiology China

基　　金：教育部高等学校博士学科点专项科研基金项目(No.2004111705);江苏省农业三项工程项目[No.SX(2007)080]资助

摘　　要：应用PCR方法从临诊疑似断奶仔猪多系统衰竭综合征(PMWS)家养野猪病例的淋巴结和脾脏中扩增出预期长度的猪圆环病毒2型(PCV2)的DNA片段,在Dulac细胞中进行分离和培养,扩增全基因组序列后进行同源性分析。结果显示,扩增产物与家猪源参考毒株的序列同源性均在98%以上,该病毒与家猪源病毒差异不大。The nucleic acid fragment of porcine circovirus type 2（PCV2）was amplified using PCR from the tissues of a domesticated wild boar with suspected postweaning multisystemic wasting syndrome （PMWS）. The sample were then inoculated into the Dulac cells and passaged for 8 generation. The 4 nucleic acid fragments covered the complete genome for PCV2 were obtained by over-lapping PCR and sent to sequence. The sequence of genome was compared with other 9 strains originated from piglets in the same area. The result showed that these strains were in high homology.

关 键 词：家养野猪 猪圆环病毒2型 分离 鉴定 

分 类 号：S852.65[农业科学—基础兽医学]