高速逆流色谱法分离纯化银杏叶中白果内酯和银杏内酯A、B、C  

Separation and purification of bilobalide and ginkgolides A,B,and C from Ginkgo biloba leaves by high-speed counter-current chromatography

作　　者：苏静[1] 谈锋[1] 李连强[1] 谢峻[1] 冯巍[1] 陈斌[1] 

机构地区：[1]西南大学生命科学学院三峡库区生态环境教育部重点实验室,重庆市三峡库区植物生态与资源重点实验室,重庆400715

出　　处：《中草药》2008年第11期1644-1648,共5页Chinese Traditional and Herbal Drugs

基　　金：三峡库区生态环境教育部重点实验室科学研究基金项目资助

摘　　要：目的从银杏叶中分离纯化白果内酯和银杏内酯A、B、C。方法银杏叶提取液经醋酸乙酯萃取、D-101大孔吸附树脂柱和Al2O3柱纯化后得到总内酯提取物,提取物再经两次高速逆流色谱分离制备4种内酯单体。结果25%乙醇热提取、醋酸乙酯萃取、D-101柱、pH4.0的Al2O3柱对总内酯的提纯最终使总内酯质量分数达到44.98%。经高速逆流色谱制备得到的白果内酯和银杏内酯A、B、C最高质量分数分别为98.3%、98.9%、98.8%、98.4%。结论本法简便快速、回收率高。Objective To separate and purify bilobalide and ginkgolide A, B, and C from Ginkgo biloba leaves. Methods Firstly the extract of G. biloba leaves was purified by extraction of acetic ether, D-101 macroporous resin, and A1203（pH 4） to obtain the crude extract of lactones. Then the crude extract was further purified by high-speed counter-current chromatography （HSCCC） to prepare the monomer of bilobalide and ginkgolide A, B, and C. Results The crude extract of laetones with the purity of 44.98% was obtained by extraction with 25% alcohol, acetic ether, D-101 macroporous resin, and Al2O3（pH 4）. When the crude extract was further purified by HSCCC, the different purity of bilobalide and ginkgolide A, B, and C was got. The maximal purity of bilobalide and ginkgolide A, B, and C was 98.3%, 98. 9%, 98.8%, and 98.4%. Conclusion This method, which is simple and rapid, provides a new way to separate and purify the lactones from G. biloba leaves.

关 键 词：银杏叶 白果内酯 银杏内酯A 银杏内酯B 银杏内酯C 高速逆流色谱 

分 类 号：R286.1[医药卫生—中药学]