大黄素对人高转移卵巢癌细胞中癌相关基因表达的影响  

Effect of emodin on cancer-related gene expression in HO-8910PM cells

作　　者：何太平[1,2] 莫丽儿[3] CHEN George-gong 梁念慈[1,3] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,广东湛江524023 [2]广东医学院检验学院,广东湛江524023 [3]广东天然药物研究与开发重点实验室,广东湛江524023 [4]香港中文大学威尔斯亲王医院外科学系

出　　处：《中草药》2008年第11期1679-1684,共6页Chinese Traditional and Herbal Drugs

基　　金：粤港科技合作项目(GHP/022/06);广东省中医药局课题(1050047);广东省科技计划项目(2007B030702003)

摘　　要：目的探讨大黄素对人高转移卵巢癌抗肿瘤的可能作用机制。方法应用肿瘤基因芯片检测大黄素(40μmol/L)对人高转移卵巢癌HO-8910PM细胞中癌相关基因表达的影响,并且实时定量PCR和Western blotting进行验证。结果共筛选出表达差异基因69个,其中33个基因表达水平上调,36个基因表达水平下调,涉及细胞凋亡、细胞周期、细胞生长与分化、细胞运动、信号转导、基因转录和细胞代谢等7大类相关基因。结论基因芯片结果提示大黄素抗肿瘤作用可能与转化生长因子-β(TGF-β)信号转导通路密切相关。Objective To explore the mechanism of antltumor ettect of emodin on highly metastatic ovarian carcinoma HO-8910PM cells. Methods The cancer-related gene expression profiles of HO- 8910PM cells with and without emodin-treatment were analyzed using genechip technology. To validate the gene chip data, some differential expression genes, including BRCA1, GDF15, and NR1D1 were analyzed by fluorescent quantitative real-time PCR and Western blotting assay. Results Sixty-nine differently expressed genes were screened out, of which up- and down-regulated genes were 33 and 36, respectively. These genes were related to apoptosis, cell cycle, cell growth and differentiation, cell motility, signal transduction, transcription, and metabolism. Conclusion The results indicats that the antitumor effect of emodin on HO-8910PM cells is involved in TGF-beta signaling pathway.

关 键 词：大黄素 卵巢癌 基因芯片 

分 类 号：R282.71[医药卫生—中药学] R963[医药卫生—中医学]